Recombinant Full Length Human IREB2 Protein, C-Flag-tagged

• Cat.No.: IREB2-516HFL
• Product Overview: Recombinant Full Length Human IREB2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 104.9 kDa
• AA Sequence: MDAPKAGYAFEYLIETLNDSSHKKF FDVSKLGTKYDVLPYSIRVLLEAAV RNCDGFLMKKEDVMNILDWK TKQSNVEVPFFPARVLLQDFTGIPA MVDFAAMREAVKTLGGDPEKVHPAC PTDLTVDHSLQIDFSKCAIQ NAPNPGGGDLQKAGKLSPLKVQPKK LPCRGQTTCRGSCDSGELGRNSGTF SSQIENTPILCPFHLQPVPE PETVLKNQEVEFGRNRERLQFFKWS SRVFKNVAVIPPGTGMAHQINLEYL SRVVFEEKDLLFPDSVVGTD SHITMVNGLGILGWGVGGIETEAVM LGLPVSLTLPEVVGCELTGSSNPFV TSIDVVLGITKHLRQVGVAG KFVEFFGSGVSQLSIVDRTTIANMC PEYGAILSFFPVDNVTLKHLEHTGF SKAKLESMETYLKAVKLFRN DQNSSGEPEYSQVIQINLNSIVPSV SGPKRPQDRVAVTDMKSDFQACLNE KVGFKGFQIAAEKQKDIVSI HYEGSEYKLSHGSVVIAAVISCTNN CNPSVMLAAGLLAKKAVEAGLRVKP YIRTSLSPGSGMVTHYLSSS GVLPYLSKLGFEIVGYGCSTCVGNT APLSDAVLNAVKQGDLVTCGILSGN KNFEGRLCDCVRANYLASPP LVVAYAIAGTVNIDFQTEPLGTDPT GKNIYLHDIWPSREEVHRVEEEHVI LSMFKALKDKIEMGNKRWNS LEAPDSVLFPWDLKSTYIRCPSFFD KLTKEPIALQAIENAHVLLYLGDSV TTDHISPAGSIARNSAAAKY LTNRGLTPREFNSYGARRGNDAVMT RGTFANIKLFNKFIGKPAPKTIHFP SGQTLDVFEAAELYQKEGIP LIILAGKKYGSGNSRDWAAKGPYLL GVKAVLAESYEKIHKDHLIGIGIAP LQFLPGENADSLGLSGRETF
  SLTFPEELSPGITLNIQTSTGKVFS VIASFEDDVEITLYKHGGLLNFVAR KFSTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: IREB2 iron responsive element binding protein 2 [ Homo sapiens (human) ]
• Official Symbol: IREB2
• Synonyms: ACO3; IRP2; IRP2AD; NDCAMA; IRE-BP2; IRE-BP 2
• Gene ID: 3658
• mRNA Refseq: NM_004136.4
• Protein Refseq: NP_004127.2
• MIM: 147582
• UniProt ID: P48200