Recombinant Full Length Human MAP2K5 Protein, GST-tagged

• Cat.No.: MAP2K5-6842HF
• Product Overview: Human MAP2K5 full-length ORF ( AAH08838, 1 a.a. - 448 a.a.) recombinant protein with GST-tag at N-terminal was expressed in wheat germ.

Specification

• Description: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 75.02 kDa
• Protein Length: 448 amino acids
• AA Sequence: MLWLALGPFPAMENQVLVIRIKIPN SGAVDWTVHSGPQLLFRDVLDVIGQ VLPEATTTAFEYEDEDGDRITVRSD EEMKAMLSYYYSTVMEQQVNGQLIE PLQIFPRACKPPGERNIHGLKVNTR AGPSQHSSPAVSDSLPSNSLKKSSA ELKKILANGQMNEQDIRYRDTLGHG NGGTVYKAYHVPSGKILAVKVILLD ITLELQKQIMSELEILYKCDSSYII GFYGAFFVENRISICTEFMDGGSLD VYRKMPEHVLGRIAVAVVKGLTYLW SLKILHRDVKPSNMLVNTRGQVKLC DFGVSTQLVNSIAKTYVGTNAYMAP ERISGEQYGIHSDVWSLGISFMELA LGRFPYPQIQKNQGSLMPLQLLQCI VDEDSPVLPVGESSEPFVHFITQCM RKQPKERPAPEELMGHPFIVQFNDG NAAVVSMWVCRALEERRSQQGPP
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: MAP2K5 mitogen-activated protein kinase kinase 5 [ Homo sapiens (human) ]
• Official Symbol: MAP2K5
• Synonyms: MAP2K5; MAPKK5; MKK5; PRKMK5; SAPKK5; SKK5; HsT17454; mitogen-activated protein kinase kinase 5; dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAPK/ERK kinase 5; MAPKK 5; MEK 5; EC 2.7.12.2
• Gene ID: 5607
• mRNA Refseq: NM_145160
• Protein Refseq: NP_660143
• MIM: 602520
• UniProt ID: Q13163