Recombinant Full Length Human ME2 Protein, C-Flag-tagged

• Cat.No.: ME2-198HFL
• Product Overview: Recombinant Full Length Human ME2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 63.4 kDa
• AA Sequence: MLSRLRVVSTTCTLACRHLHIKEKG KPLMLNPRTNKGMAFTLQERQMLGL QGLLPPKIETQDIQALRFHR NLKKMTSPLEKYIYIMGIQERNEKL FYRILQDDIESLMPIVYTPTVGLAC SQYGHIFRRPKGLFISISDR GHVRSIVDNWPENHVKAVVVTDGER ILGLGDLGVYGMGIPVGKLCLYTAC AGIRPDRCLPVCIDVGTDNI ALLKDPFYMGLYQKRDRTQQYDDLI DEFMKAITDRYGRNTLIQFEDFGNH NAFRFLRKYREKYCTFNDDI QGTAAVALAGLLAAQKVISKPISEH KILFLGAGEAALGIANLIVMSMVEN GLSEQEAQKKIWMFDKYGLL VKGRKAKIDSYQEPFTHSAPESIPD TFEDAVNILKPSTIIGVAGAGRLFT PDVIRAMASINERPVIFALS NPTAQAECTAEEAYTLTEGRCLFAS GSPFGPVKLTDGRVFTPGQGNNVYI FPGVALAVILCNTRHISDSV FLEAAKALTSQLTDEELAQGRLYPP LANIQEVSINIAIKVTEYLYANKMA FRYPEPEDKAKYVKERTWRS
  EYDSLLPDVYEWPESASSPPVITET RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Pyruvate metabolism

Gene Information

• Gene Name: ME2 malic enzyme 2 [ Homo sapiens (human) ]
• Official Symbol: ME2
• Synonyms: ODS1
• Gene ID: 4200
• mRNA Refseq: NM_002396.5
• Protein Refseq: NP_002387.1
• MIM: 154270
• UniProt ID: P23368