Recombinant Full Length Human METTL3 Protein, C-Flag-tagged

• Cat.No.: METTL3-836HFL
• Product Overview: Recombinant Full Length Human METTL3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 64.3 kDa
• AA Sequence: MSDTWSSIQAHKKQLDSLRERLQRR RKQDSGHLDLRNPEAALSPTFRSDS PVPTAPTSGGPKPSTASAVP ELATDPELEKKLLHHLSDLALTLPT DAVSICLAISTPDAPATQDGVESLL QKFAAQELIEVKRGLLQDDA HPTLVTYADHSKLSAMMGAVAEKKG PGEVAGTVTGQKRRAEQDSTTVAAF ASSLVSGLNSSASEPAKEPA KKSRKHAASDVDLEIESLLNQQSTK EQQSKKVSQEILELLNTTTAKEQSI VEKFRSRGRAQVQEFCDYGT KEECMKASDADRPCRKLHFRRIINK HTDESLGDCSFLNTCFHMDTCKYVH YEIDACMDSEAPGSKDHTPS QELALTQSVGGDSSADRLFPPQWIC CDIRYLDVSILGKFAVVMADPPWDI HMELPYGTLTDDEMRRLNIP VLQDDGFLFLWVTGRAMELGRECLN LWGYERVDEIIWVKTNQLQRIIRTG RTGHWLNHGKEHCLVGVKGN PQGFNQGLDCDVIVAEVRSTSHKPD EIYGMIERLSPGTRKIELFGRPHNV QPNWITLGNQLDGIHLLDPD
  VVARFKQRYPDGIISKPKNLTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: METTL3 methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit [ Homo sapiens (human) ]
• Official Symbol: METTL3
• Synonyms: M6A; IME4; Spo8; MT-A70; hMETTL3
• Gene ID: 56339
• mRNA Refseq: NM_019852.5
• Protein Refseq: NP_062826.2
• MIM: 612472
• UniProt ID: Q86U44