Recombinant Full Length Human MKNK2 Protein, C-Flag-tagged

• Cat.No.: MKNK2-1302HFL
• Product Overview: Recombinant Full Length Human MKNK2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 51.7 kDa
• AA Sequence: MVQKKPAELQGFHRSFKGQNPFELA FSLDQPDHGDSDFGLQCSARPDMPA SQPIDIPDAKKRGKKKKRGR ATDSFSGRFEDVYQLQEDVLGEGAH ARVQTCINLITSQEYAVKIIEKQPG HIRSRVFREVEMLYQCQGHR NVLELIEFFEEEDRFYLVFEKMRGG SILSHIHKRRHFNELEASVVVQDVA SALDFLHNKGIAHRDLKPEN ILCEHPNQVSPVKICDFDLGSGIKL NGDCSPISTPELLTPCGSAEYMAPE VVEAFSEEASIYDKRCDLWS LGVILYILLSGYPPFVGRCGSDCGW DRGEACPACQNMLFESIQEGKYEFP DKDWAHISCAAKDLISKLLV RDAKQRLSAAQVLQHPWVQGCAPEN TLPTPMVLQRNSCAKDLTSFAAEAI AMNRQLAQHDEDLAEEEAAG
  QGQPVLVRATSRCLQLSPPSQSKLA QRRQRASLSSAPVVLVGDHATRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Insulin signaling pathway, MAPK signaling pathway

Gene Information

• Gene Name: MKNK2 MAPK interacting serine/threonine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: MKNK2
• Synonyms: MNK2; GPRK7
• Gene ID: 2872
• mRNA Refseq: NM_199054.3
• Protein Refseq: NP_951009.1
• MIM: 605069
• UniProt ID: Q9HBH9