Recombinant Full Length Human NANS Protein, C-Flag-tagged

• Cat.No.: NANS-1165HFL
• Product Overview: Recombinant Full Length Human NANS Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 40.1 kDa
• AA Sequence: MPLELELCPGRWVGGQHPCFIIAEI GQNHQGDLDVAKRMIRMAKECGADC AKFQKSELEFKFNRKALERP YTSKHSWGKTYGEHKRHLEFSHDQY RELQRYAEEVGIFFTASGMDEMAVE FLHELNVPFFKVGSGDTNNF PYLEKTAKKGRPMVISSGMQSMDTM KQVYQIVKPLNPNFCFLQCTSAYPL QPEDVNLRVISEYQKLFPDI PIGYSGHETGIAISVAAVALGAKVL ERHITLDKTWKGSDHSASLEPGELA ELVRSVRLVERALGSPTKQL LPCEMACNEKLGKSVVAKVKIPEGT ILTMDMLTVKVGEPKGYPPEDIFNL VGKKVLVTVEEDDTIMEELV
  DNHGKKIKSTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways

Gene Information

• Gene Name: NANS N-acetylneuraminate synthase [ Homo sapiens (human) ]
• Official Symbol: NANS
• Synonyms: SAS; SEMDG; SEMDCG; HEL-S-100
• Gene ID: 54187
• mRNA Refseq: NM_018946.4
• Protein Refseq: NP_061819.2
• MIM: 605202
• UniProt ID: Q9NR45