Recombinant Full Length Human NARS2 Protein, C-Flag-tagged

• Cat.No.: NARS2-2110HFL
• Product Overview: Recombinant Full Length Human NARS2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of asparagine to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 24 (COXPD24).
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 53.9 kDa
• AA Sequence: MLGVRCLLRSVRFCSSAPFPKHKPS AKLSVRDALGAQNASGERIKIQGWI RSVRSQKEVLFLHVNDGSSL ESLQVVADSGLDSRELTFGSSVEVQ GQLIKSPSKRQNVELKAEKIKVIGN CDAKDFPIKYKERHPLEYLR QYPHFRCRTNVLGSILRIRSEATAA IHSFFKDSGFVHIHTPIITSNDSEG AGELFQLEPSGKLKVPEENF FNVPAFLTVSGQLHLEVMSGAFTQV FTFGPTFRAENSQSRRHLAEFYMIE AEISFVDSLQDLMQVIEELF KATTMMVLSKCPEDVELCHKFIAPG QKDRLEHMLKNNFLIISYTEAVEIL KQASQNFTFTPEWGADLRTE HEKYLVKHCGNIPVFVINYPLTLKP FYMRDNEDGPQHTVAAVDLLVPGVG ELFGGGLREERYHFLEERLA RSGLTEVYQWYLDLRRFGSVPHGGF GMGFERYLQCILGVDNIKDVIPFPR FPHSCLL TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Aminoacyl-tRNA biosynthesis

Gene Information

• Gene Name: NARS2 asparaginyl-tRNA synthetase 2, mitochondrial [ Homo sapiens (human) ]
• Official Symbol: NARS2
• Synonyms: SLM5; asnRS; DFNB94
• Gene ID: 79731
• mRNA Refseq: NM_024678.6
• Protein Refseq: NP_078954.4
• MIM: 612803
• UniProt ID: Q96I59