| Description : |
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Molecular Mass : |
118 kDa |
| AA Sequence : |
MKMASTRCKLARYLEDLEDVDLKKF KMHLEDYPPQKGCIPLPRGQTEKAD HVDLATLMIDFNGEEKAWAM AVWIFAAINRRDLYEKAKRDEPKWG SDNARVSNPTVICQEDSIEEEWMGL LEYLSRISICKMKKDYRKKY RKYVRSRFQCIEDRNARLGESVSLN KRYTRLRLIKEHRSQQEREQELLAI GKTKTCESPVSPIKMELLFD PDDEHSEPVHTVVFQGAAGIGKTIL ARKMMLDWASGTLYQDRFDYLFYIH CREVSLVTQRSLGDLIMSCC PDPNPPIHKIVRKPSRILFLMDGFD ELQGAFDEHIGPLCTDWQKAERGDI LLSSLIRKKLLPEASLLITT RPVALEKLQHLLDHPRHVEILGFSE AKRKEYFFKYFSDEAQARAAFSLIQ ENEVLFTMCFIPLVCWIVCT GLKQQMESGKSLAQTSKTTTAVYVF FLSSLLQPRGGSQEHGLCAHLWGLC SLAADGIWNQKILFEESDLR NHGLQKADVSAFLRMNLFQKEVDCE KFYSFIHMTFQEFFAAMYYLLEEEK EGRTNVPGSRLKLPSRDVTV LLENYGKFEKGYLIFVVRFLFGLVN QERTSYLEKKLSCKISQQIRLELLK WIEVKAKAKKLQIQPSQLEL FYCLYEMQEEDFVQRAMDYFPKIEI NLSTRMDHMVSSFCIENCHRVESLS LGFLHNMPKEEEEEEKEGRH LDMVQCVLPSSSHAACSHGLVNSHL TSSFCRGLFSVLSTSQSLTELDLSD NSLGDPGMRVLCETLQHPGC NIRRLWLGRCGLSHECCFDISLVLS SNQKLVELDLSDNALGDFGIRLLCV GLKHLLCNLKKLWLVSCCLT SACCQDLASVLSTSHSLTRLYVGEN ALGDSGVAILCEKAKNPQCNLQKLG LVNSGLTSVCCSALSSVLST NQNLTHLYLRGNTLGDKGIKLLCEG LLHPDCKLQVLELDNCNLTSHCCWD LSTLLTSSQSLRKLSLGNND
LGDLGVMMFCEVLKQQSCLLQNLGL SEMYFNYETKSALETLQEEKPELTV VFEPSWTRTRPLEQKLISEEDLAAN DILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome |
| Protein Pathways : |
NOD-like receptor signaling pathway |
