Recombinant Full Length Human NMNAT1 Protein, C-Flag-tagged

• Cat.No.: NMNAT1-2171HFL
• Product Overview: Recombinant Full Length Human NMNAT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 31.8 kDa
• AA Sequence: MENSEKTEVVLLACGSFNPITNMHL RLFELAKDYMNGTGRYTVVKGIISP VGDAYKKKGLIPAYHRVIMA ELATKNSKWVEVDTWESLQKEWKET LKVLRHHQEKLEASDCDHQQNSPTL ERPGRKRKWTETQDSSQKKS LEPKTKAVPKVKLLCGADLLESFAV PNLWKSEDITQIVANYGLICVTRAG NDAQKFIYESDVLWKHRSNI HVVNEWIANDISSTKIRRALRRGQS IRYLVPDLVQEYIEKHNLYSSESED RNAGVILAPLQRNTAEAKT TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism

Gene Information

• Gene Name: NMNAT1 nicotinamide nucleotide adenylyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: NMNAT1
• Synonyms: LCA9; NMNAT; PNAT1; SHILCA
• Gene ID: 64802
• mRNA Refseq: NM_022787.4
• Protein Refseq: NP_073624.2
• MIM: 608700
• UniProt ID: Q9HAN9