| Description : |
The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Molecular Mass : |
160.8 kDa |
| AA Sequence : |
MEDHMFGVQQIQPNVISVRLFKRKV GGLGFLVKERVSKPPVIISDLIRGG AAEQSGLIQAGDIILAVNGR PLVDLSYDSALEVLRGIASETHVVL ILRGPEGFTTHLETTFTGDGTPKTI RVTQPLGPPTKAVDLSHQPP AGKEQPLAVDGASGPGNGPQHAYDD GQEAGSLPHANGLAPRPPGQDPAKK ATRVSLQGRGENNELLKEIE PVLSLLTSGSRGVKGGAPAKAEMKD MGIQVDRDLDGKSHKPLPLGVENDR VFNDLWGKGNVPVVLNNPYS EKEQPPTSGKQSPTKNGSPSKCPRF LKVKNWETEVVLTDTLHLKSTLETG CTEYICMGSIMHPSQHARRP EDVRTKGQLFPLAKEFIDQYYSSIK RFGSKAHMERLEEVNKEIDTTSTYQ LKDTELIYGAKHAWRNASRC VGRIQWSKLQVFDARDCTTAHGMFN YICNHVKYATNKGNLRSAITIFPQR TDGKHDFRVWNSQLIRYAGY KQPDGSTLGDPANVQFTEICIQQGW KPPRGRFDVLPLLLQANGNDPELFQ IPPELVLEVPIRHPKFEWFK DLGLKWYGLPAVSNMLLEIGGLEFS ACPFSGWYMGTEIGVRDYCDNSRYN ILEEVAKKMNLDMRKTSSLW KDQALVEINIAVLYSFQSDKVTIVD HHSATESFIKHMENEYRCRGGCPAD WVWIVPPMSGSITPVFHQEM LNYRLTPSFEYQPDPWNTHVWKGTN GTPTKRRAIGFKKLAEAVKFSAKLM GQAMAKRVKATILYATETGK SQAYAKTLCEIFKHAFDAKVMSMEE YDIVHLEHETLVLVVTSTFGNGDPP ENGEKFGCALMEMRHPNSVQ EERKSYKVRFNSVSSYSDSQKSSGD GPDLRDNFESAGPLANVRFSVFGLG SRAYPHFCAFGHAVDTLLEE LGGERILKMREGDELCGQEEAFRTW AKKVFKAACDVFCVGDDVNIEKANN SLISNDRSWKRNKFRLTFVA EAPELTQGLSNVHKKRVSAARLLSR QNLQSPKSSRSTIFVRLHTNGSQEL QYQPGDHLGVFPGNHEDLVN ALIERLEDAPPVNQMVKVELLEERN TALGVISNWTDELRLPPCTIFQAFK YYLDITTPPTPLQLQQFASL ATSEKEKQRLLVLSKGLQEYEEWKW GKNPTIVEVLEEFPSIQMPATLLLT QLSLLQPRYYSISSSPDMYP DEVHLTVAIVSYRTRDGEGPIHHGV CSSWLNRIQADELVPCFVRGAPSFH LPRNPQVPCILVGPGTGIAP FRSFWQQRQFDIQHKGMNPCPMVLV FGCRQSKIDHIYREETLQAKNKGVF RELYTAYSREPDKPKKYVQD ILQEQLAESVYRALKEQGGHIYVCG DVTMAADVLKAIQRIMTQQGKLSAE DAGVFISRMRDDNRYHEDIF
GVTLRTYEVTNRLRSESIAFIEESK KDTDEVFSSTRTRPLEQKLISEEDL AANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome |
| Protein Pathways : |
Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer |
