Recombinant Full Length Human OGT Protein, C-Flag-tagged

• Cat.No.: OGT-1093HFL
• Product Overview: Recombinant Full Length Human OGT Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 116.7 kDa
• AA Sequence: MASSVGNVADSTEPTKRMLSFQGLA ELAHREYQAGDFEAAERHCMQLWRQ EPDNTGVLLLLSSIHFQCRR LDRSAHFSTLAIKQNPLLAEAYSNL GNVYKERGQLQEAIEHYRHALRLKP DFIDGYINLAAALVAAGDME GAVQAYVSALQYNPDLYCVRSDLGN LLKALGRLEEAKACYLKAIETQPNF AVAWSNLGCVFNAQGEIWLA IHHFEKAVTLDPNFLDAYINLGNVL KEARIFDRAVAAYLRALSLSPNHAV VHGNLACVYYEQGLIDLAID TYRRAIELQPHFPDAYCNLANALKE KGSVAEAEDCYNTALRLCPTHADSL NNLANIKREQGNIEEAVRLY RKALEVFPEFAAAHSNLASVLQQQG KLQEALMHYKEAIRISPTFADAYSN MGNTLKEMQDVQGALQCYTR AIQINPAFADAHSNLASIHKDSGNI PEAIASYRTALKLKPDFPDAYCNLA HCLQIVCDWTDYDERMKKLV SIVADQLEKNRLPSVHPHHSMLYPL SHGFRKAIAERHGNLCLDKINVLHK PPYEHPKDLKLSDGRLRVGY VSSDFGNHPTSHLMQSIPGMHNPDK FEVFCYALSPDDGTNFRVKVMAEAN HFIDLSQIPCNGKAADRIHQ DGIHILVNMNGYTKGARNELFALRP APIQAMWLGYPGTSGALFMDYIITD QETSPAEVAEQYSEKLAYMP HTFFIGDHANMFPHLKKKAVIDFKS NGHIYDNRIVLNGIDLKAFLDSLPD VKIVKMKCPDGGDNADSSNT ALNMPVIPMNTIAEAVIEMINRGQI QITINGFSISNGLATTQINNKAATG EEVPRTIIVTTRSQYGLPED AIVYCNFNQLYKIDPSTLQMWANIL KRVPNSVLWLLRFPAVGEPNIQQYA QNMGLPQNRIIFSPVAPKEE HVRRGQLADVCLDTPLCNGHTTGMD VLWAGTPMVTMPGETLASRVAASQL TCLGCLELIAKNRQEYEDIA VKLGTDLEYLKKVRGKVWKQRISSP LFNTKQYTMELERLYLQMWEHYAAG NKPDHMIKPVEVTESASGPTRTRRL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis

Gene Information

• Gene Name: OGT O-linked N-acetylglucosamine (GlcNAc) transferase [ Homo sapiens (human) ]
• Official Symbol: OGT
• Synonyms: OGT1; HRNT1; MRX106; XLID106; HINCUT-1; O-GLCNAC
• Gene ID: 8473
• mRNA Refseq: NM_181672.3
• Protein Refseq: NP_858058.1
• MIM: 300255
• UniProt ID: O15294