Recombinant Full Length Human OPA1 Protein, C-Flag-tagged

• Cat.No.: OPA1-31HFL
• Product Overview: Recombinant Full Length Human OPA1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. The encoded protein localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 109.2 kDa
• AA Sequence: MWRLRRAAVACEVCQSLVKHSSGIK GSLPLQKLHLVSRSIYHSHHPTLKL QRPQLRTSFQQFSSLTNLPL RKLKFSPIKYGYQPRRNFWPARLAT RLLKLRYLILGSAVGGGYTAKKTFD QWKDMIPDLSEYKWIVPDIV WEIDEYIDFGHKLVSEVIGASDLLL LLGSPEETAFRATDRGSESDKHFRK VSDKEKIDQLQEELLHTQLK YQRILERLEKENKELRKLVLQKDDK GIHHRKLKKSLIDMYSEVLDVLSDY DASYNTQDHLPRVVVVGDQS AGKTSVLEMIAQARIFPRGSGEMMT RSPVKVTLSEGPHHVALFKDSSREF DLTKEEDLAALRHEIELRMR KNVKEGCTVSPETISLNVKGPGLQR MVLVDLPGVINTVTSGMAPDTKETI FSISKAYMQNPNAIILCIQD GSVDAERSIVTDLVSQMDPHGRRTI FVLTKVDLAEKNVASPSRIQQIIEG KLFPMKALGYFAVVTGKGNS SESIEAIREYEEEFFQNSKLLKTSM LKAHQVTTRNLSLAVSDCFWKMVRE SVEQQADSFKATRFNLETEW KNNYPRLRELDRNELFEKAKNEILD EVISLSQVTPKHWEEILQQSLWERV STHVIENIYLPAAQTMNSGT FNTTVDIKLKQWTDKQLPNKAVEVA WETLQEEFSRFMTEPKGKEHDDIFD KLKEAVKEESIKRHKWNDFA EDSLRVIQHNALEDRSISDKQQWDA AIYFMEEALQARLKDTENAIENMVG PDWKKRWLYWKNRTQEQCVH NETKNELEKMLKCNEEHPAYLASDE ITTVRKNLESRGVEVDPSLIKDTWH QVYRRHFLKTALNHCNLCRR GFYYYQRHFVDSELECNDVVLFWRI QRMLAITANTLRQQLTNTEVRRLEK NVKEVLEDFAEDGEKKIKLL
  TGKRVQLAEDLKKVREIQEKLDAFI EALHQEKSGPTRTRRLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: OPA1 OPA1 mitochondrial dynamin like GTPase [ Homo sapiens (human) ]
• Official Symbol: OPA1
• Synonyms: NPG; NTG; MGM1; BERHS; largeG; MTDPS14
• Gene ID: 4976
• mRNA Refseq: NM_130832.3
• Protein Refseq: NP_570845.1
• MIM: 605290
• UniProt ID: E5KLK0