Recombinant Full Length Human OTUD5 Protein, C-Flag-tagged

• Cat.No.: OTUD5-2009HFL
• Product Overview: Recombinant Full Length Human OTUD5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 60.4 kDa
• AA Sequence: MTILPKKKPPPPDADPANEPPPPGP MPPAPRRGGGVGVGGGGTGVGGGDR DRDSGVVGARPRASPPPQGP LPGPPGALHRWALAVPPGAVAGPRP QQASPPPCGGPGGPGGGPGDALGAA AAGVGAAGVVVGVGGAVGVG GCCSGPGHSKRRRQAPGVGAVGGGS PEREEVGAGYNSEDEYEAAAARIEA MDPATVEQQEHWFEKALRDK KGFIIKQMKEDGACLFRAVADQVYG DQDMHEVVRKHCMDYLMKNADYFSN YVTEDFTTYINRKRKNNCHG NHIEMQAMAEMYNRPVEVYQYSTGT SAVEPINTFHGIHQNEDEPIRVSYH RNIHYNSVVNPNKATIGVGL GLPSFKPGFAEQSLMKNAIKTSEES WIEQQMLEDKKRATDWEATNEAIEE QVARESYLQWLRDQEKQARQ VRGPSQPRKASATCSSATAAASSGL EEWTSRSPRQRSSASSPEHPELHAE LGMKPPSPGTVLALAKPPSP CAPGTSSQFSAGADRATSPLVSLYP ALECRALIQQMSPSAFGLNDWDDDE ILASVLAVSQQEYLDSMKKN KVHRDPPPDKS TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Protease
• Protein Pathways: RIG-I-like receptor signaling pathway

Gene Information

• Gene Name: OTUD5 OTU deubiquitinase 5 [ Homo sapiens (human) ]
• Official Symbol: OTUD5
• Synonyms: DUBA; MCAND
• Gene ID: 55593
• mRNA Refseq: NM_017602.4
• Protein Refseq: NP_060072.1
• MIM: 300713
• UniProt ID: Q96G74