Recombinant Full Length Human PADI1 Protein, C-Flag-tagged

• Cat.No.: PADI1-1850HFL
• Product Overview: Recombinant Full Length Human PADI1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 74.5 kDa
• AA Sequence: MAPKRVVQLSLKMPTHAVCVVGVEA HVDIHSDVPKGANSFRVSGSSGVEV FMVYNRTRVKEPIGKARWPL DTDADMVVSVGTASKELKDFKVRVS YFGEQEDQALGRSVLYLTGVDISLE VDTGRTGKVKRSQGDKKTWR WGPEGYGAILLVNCDRDNHRSAEPD LTHSWLMSLADLQDMSPMLLSCNGP DKLFDSHKLVLNVPFSDSKR VRVFCARGGNSLSDYKQVLGPQCLS YEVERQPGEQEIKFYVEGLTFPDAD FLGLVSLSVSLVDPGTLPEV TLFTDTVGFRMAPWIMTPNTQPPEE LYVCRVMDTHGSNEKFLEDMSYLTL KANCKLTICPQVENRNDRWI QDEMEFGYIEAPHKSFPVVFDSPRN RGLKDFPYKRILGPDFGYVTREIPL PGPSSLDSFGNLDVSPPVTV GGTEYPLGRILIGSSFPKSGGRQMA RAVRNFLKAQQVQAPVELYSDWLSV GHVDEFLTFVPTSDQKGFRL LLASPSACLKLFQEKKEEGYGEAAQ FDGLKHQAKRSINEMLADRHLQRDN LHAQKCIDWNRNVLKRELGL AESDIVDIPQLFFLKNFYAEAFFPD MVNMVVLGKYLGIPKPYGPIINGRC CLEEKVQSLLEPLGLHCIFI DDYLSYHELQGEIHCGTNVRRKPFP FKWWNMVP TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PADI1 peptidyl arginine deiminase 1 [ Homo sapiens (human) ]
• Official Symbol: PADI1
• Synonyms: PDI; PAD1; PDI1; HPAD10
• Gene ID: 29943
• mRNA Refseq: NM_013358.3
• Protein Refseq: NP_037490.2
• MIM: 607934
• UniProt ID: Q9ULC6