Recombinant Full Length Human PASK Protein, C-Flag-tagged

• Cat.No.: PASK-1257HFL
• Product Overview: Recombinant Full Length Human PASK Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 142.7 kDa
• AA Sequence: MEDGGLTAFEEDQRCLSQSLPLPVS AEGPAAQTTAEPSRSFSSAHRHLSR RNGLSRLCQSRTALSEDRWS SYCLSSLAAQNICTSKLHCPAAPEH TDPSEPRGSVSCCSLLRGLSSGWSS PLLPAPVCNPNKAIFTVDAK TTEILVANDKACGLLGYSSQDLIGQ KLTQFFLRSDSDVVEALSEEHMEAD GHAAVVFGTVVDIISRSGEK IPVSVWMKRMRQERRLCCVVVLEPV ERVSTWVAFQSDGTVTSCDSLFAHL HGYVSGEDVAGQHITDLIPS VQLPPSGQHIPKNLKIQRSVGRARD GTTFPLSLKLKSQPSSEEATTGEAA PVSGYRASVWVFCTISGLIT LLPDGTIHGINHSFALTLFGYGKTE LLGKNITFLIPGFYSYMDLAYNSSL QLPDLASCLDVGNESGCGER TLDPWQGQDPAEGGQDPRINVVLAG GHVVPRDEIRKLMESQDIFTGTQTE LIAGGQLLSCLSPQPAPGVD NVPEGSLPVHGEQALPKDQQITALG REEPVAIESPGQDLLGESRSEPVDV KPFASCEDSEAPVPAEDGGS DAGMCGLCQKAQLERMGVSGPSGSD LWAGAAVAKPQAKGQLAGGSLLMHC PCYGSEWGLWWRSQDLAPSP SGMAGLSFGTPTLDEPWLGVENDRE ELQTCLIKEQLSQLSLAGALDVPHA ELVPTECQAVTAPVSSCDLG GRDLCGGCTGSSSACYALATDLPGG LEAVEAQEVDVNSFSWNLKELFFSD QTDQTSSNCSCATSELRETP SSLAVGSDPDVGSLQEQGSCVLDDR ELLLLTGTCVDLGQGRRFRESCVGH DPTEPLEVCLVSSEHYAASD RESPGHVPSTLDAGPEDTCPSAEEP RLNVQVTSTPVIVMRGAAGLQREIQ EGAYSGSCYHRDGLRLSIQF EVRRVELQGPTPLFCCWLVKDLLHS QRDSAARTRLFLASLPGSTHSTAAE LTGPSLVEVLRARPWFEEPP KAVELEGLAACEGEYSQKYSTMSPL GSGAFGFVWTAVDKEKNKEVVVKFI KKEKVLEDCWIEDPKLGKVT LEIAILSRVEHANIIKVLDIFENQG FFQLVMEKHGSGLDLFAFIDRHPRL DEPLASYIFRQLVSAVGYLR LKDIIHRDIKDENIVIAEDFTIKLI DFGSAAYLERGKLFYTFCGTIEYCA PEVLMGNPYRGPELEMWSLG VTLYTLVFEENPFCELEETVEAAIH PPYLVSKELMSLVSGLLQPVPERRT TLEKLVTDPWVTQPVNLADY
  TWEEVFRVNKPESGVLSAASLEMGN RSLSDVAQAQELCGGPVPGEAPNGQ GCLHPGDPRLLTSTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency

Gene Information

• Gene Name: PASK PAS domain containing serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: PASK
• Synonyms: STK37; PASKIN
• Gene ID: 23178
• mRNA Refseq: NM_015148.4
• Protein Refseq: NP_055963.2
• MIM: 607505
• UniProt ID: Q96RG2