Recombinant Full Length Human PCMT1 Protein, C-Flag-tagged

• Cat.No.: PCMT1-1978HFL
• Product Overview: Recombinant Full Length Human PCMT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 30.1 kDa
• AA Sequence: MAWKSGGASHSELIHNLRKNGIIKT DKVFEVMLATDRSHYAKCNPYMDSP QSIGFQATISAPHMHAYALE LLFDQLHEGAKALDVGSGSGILTAC FARMVGCTGKVIGIDHIKELVDDSI NNVRKDDPTLLSSGRVQLVV GDGRMGYAEEAPYDAIHVGAAAPVV PQALIDQLKPGGRLILPVGPAGGNQ MLEQYDKLQDGSIKMKPLMG VIYVPLTDKEKQWSRWK TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: PCMT1 protein-L-isoaspartate (D-aspartate) O-methyltransferase [ Homo sapiens (human) ]
• Official Symbol: PCMT1
• Synonyms: PIMT
• Gene ID: 5110
• mRNA Refseq: NM_005389.2
• Protein Refseq: NP_005380.2
• MIM: 176851
• UniProt ID: P22061