Recombinant Full Length Human PDE11A Protein, C-Flag-tagged

• Cat.No.: PDE11A-1908HFL
• Product Overview: Recombinant Full Length Human PDE11A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 104.6 kDa
• AA Sequence: MAASRLDFGEVETFLDRHPELFEDY LMRKGKQEMVEKWLQRHSQGQGALG PRPSLAGTSSLAHSTCRGGS SVGGGTGPNGSAHSQPLPGGGDCGG VPLSPSWAGGSRGDGNLQRRASQKE LRKSFARSKAIHVNRTYDEQ VTSRAQEPLSSVRRRALLRKASSLP PTTAHILSALLESRVNLPQYPPTAI DYKCHLKKHNERQFFLELVK DISNDLDLTSLSYKILIFVCLMVDA DRCSLFLVEGAAAGKKTLVSKFFDV HAGTPLLPCSSTENSNEVQV PWGKGIIGYVGEHGETVNIPDAYQD RRFNDEIDKLTGYKTKSLLCMPIRS SDGEIIGVAQAINKIPEGAP FTEDDEKVMQMYLPFCGIAISNAQL FAASRKEYERSRALLEVVNDLFEEQ TDLEKIVKKIMHRAQTLLKC ERCSVLLLEDIESPVVKFTKSFELM SPKCSADAENSFKESMEKSSYSDWL INNSIAELVASTGLPVNISD AYQDPRFDAEADQISGFHIRSVLCV PIWNSNHQIIGVAQVLNRLDGKPFD DADQRLFEAFVIFCGLGINN TIMYDQVKKSWAKQSVALDVLSYHA TCSKAEVDKFKAANIPLVSELAIDD IHFDDFSLDVDAMITAALRM FMELGMVQKFKIDYETLCRWLLTVR KNYRMVLYHNWRHAFNVCQLMFAML TTAGFQDILTEVEILAVIVG CLCHDLDHRGTNNAFQAKSGSALAQ LYGTSATLEHHHFNHAVMILQSEGH NIFANLSSKEYSDLMQLLKQ SILATDLTLYFERRTEFFELVSKGE YDWNIKNHRDIFRSMLMTACDLGAV TKPWEISRQVAELVTSEFFE QGDRERLELKLTPSAIFDRNRKDEL PRLQLEWIDSICMPLYQALVKVNVK LKPMLDSVATNRSKWEELHQ KRLLASTASSSPASVMVAKEDRN TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism

Gene Information

• Gene Name: PDE11A phosphodiesterase 11A [ Homo sapiens (human) ]
• Official Symbol: PDE11A
• Synonyms: PPNAD2
• Gene ID: 50940
• mRNA Refseq: NM_016953.4
• Protein Refseq: NP_058649.3
• MIM: 604961
• UniProt ID: Q9HCR9