Recombinant Full Length Human PHGDH Protein, C-Flag-tagged

• Cat.No.: PHGDH-1414HFL
• Product Overview: Recombinant Full Length Human PHGDH Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 56.5 kDa
• AA Sequence: MAFANLRKVLISDSLDPCCRKILQD GGLQVVEKQNLSKEELIAELQDCEG LIVRSATKVTADVINAAEKL QVVGRAGTGVDNVDLEAATRKGILV MNTPNGNSLSAAELTCGMIMCLARQ IPQATASMKDGKWERKKFMG TELNGKTLGILGLGRIGREVATRMQ SFGMKTIGYDPIISPEVSASFGVQQ LPLEEIWPLCDFITVHTPLL PSTTGLLNDNTFAQCKKGVRVVNCA RGGIVDEGALLRALQSGQCAGAALD VFTEEPPRDRALVDHENVIS CPHLGASTKEAQSRCGEEIAVQFVD MVKGKSLTGVVNAQALTSAFSPHTK PWIGLAEALGTLMRAWAGSP KGTIQVITQGTSLKNAGNCLSPAVI VGLLKEASKQADVNLVNAKLLVKEA GLNVTTSHSPAAPGEQGFGE CLLAVALAGAPYQAVGLVQGTTPVL QGLNGAVFRPEVPLRRDLPLLLFRT QTSDPAMLPTMIGLLAEAGV
  RLLSYQTSLVSDGETWHVMGISSLL PSLEAWKQHVTEAFQFHFTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways

Gene Information

• Gene Name: PHGDH phosphoglycerate dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: PHGDH
• Synonyms: NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
• Gene ID: 26227
• mRNA Refseq: NM_006623.4
• Protein Refseq: NP_006614.2
• MIM: 606879
• UniProt ID: O43175