Recombinant Full Length Human PHLPP1 Protein, C-Flag-tagged

• Cat.No.: PHLPP1-1697HFL
• Product Overview: Recombinant Full Length Human PHLPP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 184.5 kDa
• AA Sequence: MEPAAAATVQRLPELGREDRASAPA AAAAAAAAAAAAAAALAAAAGGGRS PEPALTPAAPSGGNGSGSGAREEAP GEAPPGPLPGRAGGAGRRRRRGAPQ PIAGGAAPVPGAGGGANSLLLRRGR LKRNLSAAAAAASSSSSSSAAAASH SPGAAGLPASCSASASLCTRSLDRK TLLLKHRQTLQLQPSDRDWVRHQLQ RGCVHVFDRHMASTYLRPVLCTLDT TAGEVAARLLQLGHKGGGVVKVLGQ GPGAAAAREPAEPPPEAGPRLAPPE PRDSEVPPARSAPGAFGGPPRAPPA DLPLPVGGPGGWSRRASPAPSDSSP GEPFVGGPVSSPRAPRPVVSDTESF SLSPSAESVSDRLDPYSSGGGSSSS SEELEADAASAPTGVPGQPRRPGHP AQPLPLPQTASSPQPQQKAPRAIDS PGGAVREGSCEEKAAAAVAPGGLQS TPGRSGVTAEKAPPPPPPPTLYVQL HGETTRRLEAEEKPLQIQNDYLFQL GFGELWRVQEEGMDSEIGCLIRFYA GKPHSTGSSERIQLSGMYNVRKGKM QLPVNRWTRRQVILCGTCLIVSSVK DSLTGKMHVLPLIGGKVEEVKKHQH CLAFSSSGPQSQTYYICFDTFTEYL RWLRQVSKVASQRISSVDLSCCSLE HLPANLFYSQDLTHLNLKQNFLRQN PSLPAARGLNELQRFTKLKSLNLSN NHLGDFPLAVCSIPTLAELNVSCNA LRSVPAAVGVMHNLQTFLLDGNFLQ SLPAELENMKQLSYLGLSFNEFTDI PEVLEKLTAVDKLCMSGNCVETLRL QALRKMPHIKHVDLRLNVIRKLIAD EVDFLQHVTQLDLRDNKLGDLDAMI FNNIEVLHCERNQLVTLDICGYFLK ALYASSNELVQLDVYPVPNYLSYMD VSRNRLENVPEWVCESRKLEVLDIG HNQICELPARLFCNSSLRKLLAGHN QLARLPERLERTSVEVLDVQHNQLL ELPPNLLMKADSLRFLNASANKLES LPPATLSEETNSILQELYLTNNSLT DKCVPLLTGHPHLKILHMAYNRLQS FPASKMAKLEELEEIDLSGNKLKAI PTTIMNCRRMHTVIAHSNCIEVFPE VMQLPEIKCVDLSCNELSEVTLPEN LPPKLQELDLTGNPRLVLDHKTLEL LNNIRCFKIDQPSTGDASGAPAVWS HGYTEASGVKNKLCVAALSVNNFCD NREALYGVFDGDRNVEVPYLLQCTM SDILAEELQKTKNEEEYMVNTFIVM QRKLGTAGQKLGGAAVLCHIKHDPV DPGGSFTLTSANVGKCQTVLCRNGK PLPLSRSYIMSCEEELKRIKQHKAI ITEDGKVNGVTESTRILGYTFLHPS VVPRPHVQSVLLTPQDEFFILGSKG LWDSLSVEEAVEAVRNVPDALAAAK KLCTLAQSYGCHDSISAVVVQLSVT EDSFCCCELSAGGAVPPPSPGIFPP SVNMVIKDRPSDGLGVPSSSSGMAS EISSELSTSEMSSEVGSTASDEPPP GALSENSPAYPSEQRCMLHPICLSN SFQRQLSSATFSSAFSDNGLDSDDE EPIEGVFTNGSRVEVEVDIHCSRAK EKEKQQHLLQVPAEASDEGIVISAN EDEPGLPRKADFSAVGTIGRRRANG SVAPQERSHNVIEVATDAPLRKPGG YFAAPAQPDPDDQFIIPPELEEEVK EIMKHHQEQQQQQQPPPPPQLQPQL PRHYQLDQLPDYYDTPLTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PHLPP1 PH domain and leucine rich repeat protein phosphatase 1 [ Homo sapiens (human) ]
• Official Symbol: PHLPP1
• Synonyms: SCOP; PHLPP; PPM3A; PLEKHE1
• Gene ID: 23239
• mRNA Refseq: NM_194449.4
• Protein Refseq: NP_919431.2
• MIM: 609396
• UniProt ID: O60346