Recombinant Full Length Human Phosphoserine Phosphatase / PSPH Protein, Untagged

• Cat.No.: PSPH-198H
• Product Overview: Recombinant human Phosphoserine Phosphatase was overexpressed inE. coliand purified by conventional chromatography. MW = 25 kDa (225 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-225 a.a.
• Description: Human Phosphoserine phosphatase (hPSP) is an important enzyme in the phosphorylated pathway of serine biosynthesis, which contributes a major portion of the endogenous L-serine. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2+-dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. Recently, its complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the -helical bundle domain, is important to substrate recognition and hydrolysis.
• Form: Liquid. in 20 mM HEPES( pH 7.5) containing 1 mM DTT, 100 mM KCl2.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Sequences Of Amino Acids: MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AM G GA VPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPGIRELVSRLQERNVQVF LI SG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK V I KLLKEKFH FKKIIMIGDGATDMEACPPA DAFIGFGGNV IRQQVKDNAK WY IT D F VELL GELEE.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PSPH phosphoserine phosphatase [ Homo sapiens ]
• Synonyms: PSPH; phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrol ase; L-3-phosphoserine phosphatase; EC 3.1.3.3
• Gene ID: 5723
• mRNA Refseq: NM_004577
• Protein Refseq: NP_004568
• MIM: 172480
• UniProt ID: P78330
• Chromosome Location: 7p11.2
• Pathway: Glycine, serine and threonine metabolism; Metabolic pathways
• Function: hydrolase activity; magnesium ion binding; phosphoserine phosphatase activity; protein binding