Recombinant Full Length Human PLA2G15 Protein, C-Flag-tagged

• Cat.No.: PLA2G15-409HFL
• Product Overview: Recombinant Full Length Human PLA2G15 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46.5 kDa
• AA Sequence: MGLHLRPYRVGLLPDGLLFLLLLLM LLADPALPAGRHPPVVLVPGDLGNQ LEAKLDKPTVVHYLCSKKTE SYFTIWLNLELLLPVIIDCWIDNIR LVYNKTSRATQFPDGVDVRVPGFGK TFSLEFLDPSKSSVGSYFHT MVESLVGWGYTRGEDVRGAPYDWRR APNENGPYFLALREMIEEMYQLYGG PVVLVAHSMGNMYTLYFLQR QPQAWKDKYIRAFVSLGAPWGGVAK TLRVLASGDNNRIPVIGPLKIREQQ RSAVSTSWLLPYNYTWSPEK VFVQTPTINYTLRDYRKFFQDIGFE DGWLMRQDTEGLVEATMPPGVQLHC LYGTGVPTPDSFYYESFPDR
  DPKICFGDGDGTVNLKSALQCQAWQ SRQEHQVLLQELPGSEHIEMLANAT TLAYLKRVLLGPTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Secreted Protein
• Protein Pathways: Glycerophospholipid metabolism, Lysosome

Gene Information

• Gene Name: PLA2G15 phospholipase A2 group XV [ Homo sapiens (human) ]
• Official Symbol: PLA2G15
• Synonyms: ACS; LLPL; LPLA2; LYPLA3; GXVPLA2
• Gene ID: 23659
• mRNA Refseq: NM_012320.4
• Protein Refseq: NP_036452.1
• MIM: 609362
• UniProt ID: Q8NCC3