Recombinant Full Length Human PON1 Protein, C-Flag-tagged

• Cat.No.: PON1-499HFL
• Product Overview: Recombinant Full Length Human PON1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 39.6 kDa
• AA Sequence: MAKLIALTLLGMGLALFRNHQSSYQ TRLNALREVQPVELPNCNLVKGIET GSEDLEILPNGLAFISSGLK YPGIKSFNPNSPGKILLMDLNEEDP TVLELGITGSKFDVSSFNPHGISTF TDEDNAMYLLVVNHPDAKST VELFKFQEEEKSLLHLKTIRHKLLP NLNDIVAVGPEHFYGTNDHYFLDPY LQSWEMYLGLAWSYVVYYSP SEVRVVAEGFDFANGINISPDGKYV YIAELLAHKIHVYEKHANWTLTPLK SLDFNTLVDNISVDPETGDL WVGCHPNGMKIFFYDSENPPASEVL RIQNILTEEPKVTQVYAENGTVLQG STVASVYKGKLLIGTVFHKA
  LYCELTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Metabolic pathways

Gene Information

• Gene Name: PON1 paraoxonase 1 [ Homo sapiens (human) ]
• Official Symbol: PON1
• Synonyms: ESA; PON; MVCD5
• Gene ID: 5444
• mRNA Refseq: NM_000446.7
• Protein Refseq: NP_000437.3
• MIM: 168820
• UniProt ID: P27169