Recombinant Full Length Human PPP1CB Protein, C-Flag-tagged

• Cat.No.: PPP1CB-755HFL
• Product Overview: Recombinant Full Length Human PPP1CB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37 kDa
• AA Sequence: MADGELNVDSLITRLLEVRGCRPGK IVQMTEAEVRGLCIKSREIFLSQPI LLELEAPLKICGDIHGQYTD LLRLFEYGGFPPEANYLFLGDYVDR GKQSLETICLLLAYKIKYPENFFLL RGNHECASINRIYGFYDECK RRFNIKLWKTFTDCFNCLPIAAIVD EKIFCCHGGLSPDLQSMEQIRRIMR PTDVPDTGLLCDLLWSDPDK DVQGWGENDRGVSFTFGADVVSKFL NRHDLDLICRAHQVVEDGYEFFAKR QLVTLFSAPNYCGEFDNAGG
  MMSVDETLMCSFQILKPSEKKAKYQ YGGLNSGRPVTPPRTANPPKKRTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Long-term potentiation, Oocyte meiosis, Regulation of actin cytoskeleton, Vascular smooth muscle contraction

Gene Information

• Gene Name: PPP1CB protein phosphatase 1 catalytic subunit beta [ Homo sapiens (human) ]
• Official Symbol: PPP1CB
• Synonyms: MP; PP1B; PP1c; NSLH2; PP-1B; PPP1CD; PP1beta; PPP1beta; HEL-S-80p
• Gene ID: 5500
• mRNA Refseq: NM_206876.2
• Protein Refseq: NP_996759.1
• MIM: 600590
• UniProt ID: P62140