Recombinant Full Length Human PRAG1 Protein, C-Flag-tagged

• Cat.No.: PRAG1-1026HFL
• Product Overview: Recombinant Full Length Human PRAG1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA).
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 149.1 kDa
• AA Sequence: MHQTLCLNPESLKMSACSDFVEHIW KPGSCKNCFCLRSDHQLVAGPPQPR AGSLPPPPRLPPRPENCRLE DEGVNSSPYSKPTIAVKPTMMSSEA SDVWTEANLSAEVSQVIWRRAPGKL PLPKQEDAPVVYLGSFRGVQ KPAGPSTSPDGNSRCPPAYTMVGLH NLEPRGERNIAFHPVSFPEEKAVHK EKPSFPYQDRPSTQESFRQK LAAFAGTTSGCHQGPGPLRESLPSE DDSDQRCSPSGDSEGGEYCSILDCC PGSPVAKAASQTAGSRGRHG GRDCSPTCWEQGKCSGPAEQEKRGP SFPKECCSQGPTAHPSCLGPKKLSL TSEAAISSDGLSCGSGSGSG ASSPFVPHLESDYCSLMKEPAPEKQ QDPGCPGVTPSRCLGLTGEPQPPAH PREATQPEPIYAESTKRKKA APVPSKSQAKIEHAAAAQGQGQVCT GNAWAQKAASGWGRDSPDPTPQVSA TITVMAAHPEEDHRTIYLSS PDSAVGVQWPRGPVSQNSEVGEEET SAGQGLSSRESHAHSASESKPKERP AIPPKLSKSSPVGSPVSPSA GGPPVSPLADLSDGSSGGSSIGPQP PSQGPADPAPSCRTNGVAISDPSRC PQPAASSASEQRRPRFQAGT WSRQCRIEEEEEVEQELLSHSWGRE TKNGPTDHSNSTTWHRLHPTDGSSG QNSKVGTGMSKSASFAFEFP KDRSGIETFSPPPPPPKSRHLLKMN KSSSDLEKVSQGSAESLSPSFRGVH VSFTTGSTDSLASDSRTCSD GGPSSELAHSPTNSGKKLFAPVPFP SGSTEDVSPSGPQQPPPLPQKKIVS RAASSPDGFFWTQGSPKPGT ASPKLNLSHSETNVHDESHFSYSLS PGNRHHPVFSSSDPLEKAFKGSGHW LPAAGLAGNRGGCGSPGLQC KGAPSASSSQLSVSSQASTGSTQLQ LHGLLSNISSKEGTYAKLGGLYTQS LARLVAKCEDLFMGGQKKEL HFNENNWSLFKLTCNKPCCDSGDAI YYCATCSEDPGSTYAVKICKAPEPK TVSYCSPSVPVHFNIQQDCG HFVASVPSSMLSSPDAPKDPVPALP THPPAQEQDCVVVITREVPHQTASD FVRDSAASHQAEPEAYERRV CFLLLQLCNGLEHLKEHGIIHRDLC LENLLLVHCTLQAGPGPAPAPAPAP AAAAPPCSSAAPPAGGTLSP AAGPASPEGPREKQLPRLIISNFLK AKQKPGGTPNLQQKKSQARLAPEIV SASQYRKFDEFQTGILIYEL LHQPNPFEVRAQLRERDYRQEDLPP LPALSLYSPGLQQLAHLLLEADPIK RIRIGEAKRVLQCLLWGPRR ELVQQPGTSEEALCGTLHNWIDMKR ALMMMKFAEKAVDRRRGVELEDWLC CQYLASAEPGALLQSLKLLQ
  LLTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Protein Kinase

Gene Information

• Gene Name: PRAG1 PEAK1 related, kinase-activating pseudokinase 1 [ Homo sapiens (human) ]
• Official Symbol: PRAG1
• Synonyms: NACK; PEAK2; SGK223; PRAGMIN
• Gene ID: 157285
• mRNA Refseq: NM_001080826.3
• Protein Refseq: NP_001074295.2
• MIM: 617344
• UniProt ID: Q86YV5