Recombinant Full Length Human PTPRB Protein, C-Flag-tagged

• Cat.No.: PTPRB-744HFL
• Product Overview: Recombinant Full Length Human PTPRB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and one intracytoplasmic catalytic domain, thus belongs to receptor type PTP. The extracellular region of this PTP is composed of multiple fibronectin type_III repeats, which was shown to interact with neuronal receptor and cell adhesion molecules, such as contactin and tenascin C. This protein was also found to interact with sodium channels, and thus may regulate sodium channels by altering tyrosine phosphorylation status. The functions of the interaction partners of this protein implicate the roles of this PTP in cell adhesion, neurite growth, and neuronal differentiation. Alternate transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 249 kDa
• AA Sequence: MEAEFYMVILTCLIFRNSEGFQIVH VQKQQCLFKNEKVVVGSCNRTIQNQ QWMWTEDEKLLHVKSALCLA ISNSSRGPSRSAILDRCSQAPRWTC YDQEGFLEVENASLFLQKQGSRVVV KKARKYLHSWMKIDVNKEGK LVNESLCLQKAGLGAEVSVRSTRNT APPQILTTFNAVPDGLVFLIRNTTE AFIRNAAENYSQNSSERQHP NLHMTGITDTSWVLSTTQPFSSTTE ETGLAEPERCNFTLAESKASSHSVS IQWRILGSPCNFSLIYSSDT LGAALCPTFRIDNTTYGCNLQDLQA GTIYNFRIISLDEERTVVLQTDPLP PARFGVSKEKTTSTSLHVWW TPSSGKVTSYEVQLFDENNQKIQGV QIQESTSWNEYTFFNLTAGSKYNIA ITAVSGGKRSFSVYTNGSTV PSPVKDIGISTKANSLLISWSHGSG NVERYRLMLMDKGILVHGGVVDKHA TSYAFHGLTPGYLYNLTVMT EAAGLQNYRWKLVRTAPMEVSNLKV TNDGSLTSLKVKWQRPPGNVDSYNI TLSHKGTIKESRVLAPWITE THFKELVPGRLYQVTVSCVSGELSA QKMAVGRTFPDKVANLEANNNGRMR SLVVSWSPPAGDWEQYRILL FNDSVVLLNITVGKEETQYVMDDTG LVPGRQYEVEVIVESGNLKNSERCQ GRTVPLAVLQLRVKHANETS LSIMWQTPVAEWEKYIISLADRDLL LIHKSLSKDAKEFTFTDLVPGRKYM ATVTSISGDLKNSSSVKGRT VPAQVTDLHVANQGMTSSLFTNWTQ AQGDVEFYQVLLIHENVVIKNESIS SETSRYSFHSLKSGSLYSVV VTTVSGGISSRQVVVEGRTVPSSVS GVTVNNSGRNDYLSVSWLLAPGDVD NYEVTLSHDGKVVQSLVIAK SVRECSFSSLTPGRLYTVTITTRSG KYENHSFSQERTVPDKVQGVSVSNS ARSDYLRVSWVHATGDFDHY EVTIKNKNNFIQTKSIPKSENECVF VQLVPGRLYSVTVTTKSGQYEANEQ GNGRTIPEPVKDLTLRNRST EDLHVTWSGANGDVDQYEIQLLFND MKVFPPFHLVNTATEYRFTSLTPGR QYKILVLTISGDVQQSAFIE GFTVPSAVKNIHISPNGATDSLTVN WTPGGGDVDSYTVSAFRHSQKVDSQ TIPKHVFEHTFHRLEAGEQY QIMIASVSGSLKNQINVVGRTVPAS VQGVIADNAYSSYSLIVSWQKAAGV AERYDILLLTENGILLRNTS EPATTKQHKFEDLTPGKKYKIQILT VSGGLFSKEAQTEGRTVPAAVTDLR ITENSTRHLSFRWTASEGEL SWYNIFLYNPDGNLQERAQVDPLVQ SFSFQNLLQGRMYKMVIVTHSGELS NESFIFGRTVPASVSHLRGS NRNTTDSLWFNWSPASGDFDFYELI LYNPNGTKKENWKDKDLTEWRFQGL VPGRKYVLWVVTHSGDLSNK VTAESRTAPSPPSLMSFADIANTSL AITWKGPPDWTDYNDFELQWLPRDA LTVFNPYNNRKSEGRIVYGL RPGRSYQFNVKTVSGDSWKTYSKPI FGSVRTKPDKIQNLHCRPQNSTAIA CSWIPPDSDFDGYSIECRKM DTQEVEFSRKLEKEKSLLNIMMLVP HKRYLVSIKVQSAGMTSEVVEDSTI TMIDRPPPPPPHIRVNEKDV LISKSSINFTVNCSWFSDTNGAVKY FTVVVREADGSDELKPEQQHPLPSY LEYRHNASIRVYQTNYFASK CAENPNSNSKSFNIKLGAEMESLGG KCDPTQQKFCDGPLKPHTAYRISIR AFTQLFDEDLKEFTKPLYSD TFFSLPITTESEPLFGAIEGVSAGL FLIGMLVAVVALLICRQKVSHGRER PSARLSIRRDRPLSVHLNLG QKGNRKTSCPIKINQFEGHFMKLQA DSNYLLSKEYEELKDVGRNQSCDIA LLPENRGKNRYNNILPYDAT RVKLSNVDDDPCSDYINASYIPGNN FRREYIVTQGPLPGTKDDFWKMVWE QNVHNIVMVTQCVEKGRVKC DHYWPADQDSLYYGDLILQMLSESV LPEWTIREFKICGEEQLDAHRLIRH FHYTVWPDHGVPETTQSLIQ FVRTVRDYINRSPGAGPTVVHCSAG VGRTGTFIALDRILQQLDSKDSVDI YGAVHDLRLHRVHMVQTECQ
  YVYLHQCVRDVLRARKLRSEQENPL FPIYENVNPEYHRDPVYSRHTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Adherens junction

Gene Information

• Gene Name: PTPRB protein tyrosine phosphatase receptor type B [ Homo sapiens (human) ]
• Official Symbol: PTPRB
• Synonyms: PTPB; HPTPB; VEPTP; HPTP-BETA; R-PTP-BETA
• Gene ID: 5787
• mRNA Refseq: NM_001109754.4
• Protein Refseq: NP_001103224.1
• MIM: 176882
• UniProt ID: P23467