Recombinant Full Length Human PUM1 Protein, C-Flag-tagged

• Cat.No.: PUM1-930HFL
• Product Overview: Recombinant Full Length Human PUM1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 126.3 kDa
• AA Sequence: MSVACVLKRKAVLWQDSFSPHLKHH PQEPANPNMPVVLTSGTGSQAQPQP AANQALAAGTHSSPVPGSIG VAGRSQDDAMVDYFFQRQHGEQLGG GGSGGGGYNNSKHRWPTGDNIHAEH QVRSMDELNHDFQALALEGR AMGEQLLPGKKFWETDESSKDGPKG IFLGDQWRDSAWGTSDHSVSQPIMV QRRPGQSFHVNSEVNSVLSP RSESGGLGVSMVEYVLSSSPGDSCL RKGGFGPRDADSDENDKGEKKNKGT FDGDKLGDLKEEGDVMDKTN GLPVQNGIDADVKDFSRTPGNCQNS ANEVDLLGPNQNGSEGLAQLTSTNG AKPVEDFSNMESQSVPLDPM EHVGMEPLQFDYSGTQVPVDSAAAT VGLFDYNSQQQLFQRPNALAVQQLT AAQQQQYALAAAHQPHIGLA PAAFVPNPYIISAAPPGTDPYTAGL AAAATLGPAVVPHQYYGVTPWGVYP ASLFQQQAAAAAAATNSANQ QTTPQAQQGQQQVLRGGASQRPLTP NQNQQGQQTDPLVAAAAVNSALAFG QGLAAGMPGYPVLAPAAYYD QTGALVVNAGARNGLGAPVRLVAPA PVIISSSAAQAAVAAAAASANGAAG GLAGTTNGPFRPLGTQQPQP QPQQQPNNNLASSSFYGNNSLNSNS QSSSLFSQGSAQPANTSLGFGSSSS LGATLGSALGGFGTAVANSN TGSGSRRDSLTGSSDLYKRTSSSLT PIGHSFYNGLSFSSSPGPVGMPLPS QGPGHSQTPPPSLSSHGSSS SLNLGGLTNGSGRYISAAPGAEAKY RSASSASSLFSPSSTLFSSSRLRYG MSDVMPSGRSRLLEDFRNNR YPNLQLREIAGHIMEFSQDQHGSRF IQLKLERATPAERQLVFNEILQAAY QLMVDVFGNYVIQKFFEFGS LEQKLALAERIRGHVLSLALQMYGC RVIQKALEFIPSDQQNEMVRELDGH VLKCVKDQNGNHVVQKCIEC VQPQSLQFIIDAFKGQVFALSTHPY GCRVIQRILEHCLPDQTLPILEELH QHTEQLVQDQYGNYVIQHVL EHGRPEDKSKIVAEIRGNVLVLSQH KFASNVVEKCVTHASRTERAVLIDE VCTMNDGPHSALYTMMKDQY ANYVVQKMIDVAEPGQRKIVMHKIR PHIATLRKYTYGKHILAKLEKYYMK NGVDLGPICGPPNGIITRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PUM1 pumilio RNA binding family member 1 [ Homo sapiens (human) ]
• Official Symbol: PUM1
• Synonyms: PUMH; HSPUM; PUMH1; PUML1; SCA47
• Gene ID: 9698
• mRNA Refseq: NM_014676.3
• Protein Refseq: NP_055491.1
• MIM: 607204
• UniProt ID: Q14671