| Description : |
This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
126.5 kDa |
| AA Sequence : |
MSVACVLKRKAVLWQDSFSPHLKHH PQEPANPNMPVVLTSGTGSQAQPQP AANQALAAGTHSSPVPGSIGVAGRS QDDAMVDYFFQRQHGEQLGGGGSGG GGYNNSKHRWPTGDNIHAEHQVRSM DELNHDFQALALEGRAMGEQLLPGK KFWETDESSKDGPKGIFLGDQWRDS AWGTSDHSVSQPIMVQRRPGQSFHV NSEVNSVLSPRSESGGLGVSMVEYV LSSSPGDSCLRKGGFGPRDADSDEN DKGEKKNKGTFDGDKLGDLKEEGDV MDKTNGLPVQNGIDADVKDFSRTPG NCQNSANEVDLLGPNQNGSEGLAQL TSTNGAKPVEDFSNMESQSVPLDPM EHVGMEPLQFDYSGTQVPVDSAAAT VGLFDYNSQQQLFQRPNALAVQQLT AAQQQQYALAAAHQPHIGLAPAAFV PNPYIISAAPPGTDPYTAGLAAAAT LGPAVVPHQYYGVTPWGVYPASLFQ QQAAAAAAATNSANQQTTPQAQQGQ QQVLRGGASQRPLTPNQNQQGQQTD PLVAAAAVNSALAFGQGLAAGMPGY PVLAPAAYYDQTGALVVNAGARNGL GAPVRLVAPAPVIISSSAAQAAVAA AAASANGAAGGLAGTTNGPFRPLGT QQPQPQPQQQPNNNLASSSFYGNNS LNSNSQSSSLFSQGSAQPANTSLGF GSSSSLGATLGSALGGFGTAVANSN TGSGSRRDSLTGSSDLYKRTSSSLT PIGHSFYNGLSFSSSPGPVGMPLPS QGPGHSQTPPPSLSSHGSSSSLNLG GLTNGSGRYISAAPGAEAKYRSASS ASSLFSPSSTLFSSSRLRYGMSDVM PSGRSRLLEDFRNNRYPNLQLREIA GHIMEFSQDQHGSRFIQLKLERATP AERQLVFNEILQAAYQLMVDVFGNY VIQKFFEFGSLEQKLALAERIRGHV LSLALQMYGCRVIQKALEFIPSDQQ NEMVRELDGHVLKCVKDQNGNHVVQ KCIECVQPQSLQFIIDAFKGQVFAL STHPYGCRVIQRILEHCLPDQTLPI LEELHQHTEQLVQDQYGNYVIQHVL EHGRPEDKSKIVAEIRGNVLVLSQH KFASNVVEKCVTHASRTERAVLIDE VCTMNDGPHSALYTMMKDQYANYVV QKMIDVAEPGQRKIVMHKIRPHIAT LRKYTYGKHILAKLEKYYMKNGVDL GPICGPPNGIITRTRPLEQKLISEE DLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
