Recombinant Full Length Human PUS7 Protein, C-Flag-tagged

• Cat.No.: PUS7-771HFL
• Product Overview: Recombinant Full Length Human PUS7 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables enzyme binding activity and pseudouridine synthase activity. Involved in several processes, including pseudouridine synthesis; regulation of hematopoietic stem cell differentiation; and regulation of mesoderm development. Located in nucleus.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 74.9 kDa
• AA Sequence: MEMTEMTGVSLKRGALVVEDNDSGV PVEETKKQKLSECSLTKGQDGLQND FLSISEDVPRPPDTVSTGKG GKNSEAQLEDEEEEEEDGLSEECEE EESESFADMMKHGLTEADVGITKFV SSHQGFSGILKERYSDFVVH EIGKDGRISHLNDLSIPVDEEDPSE DIFTVLTAEEKQRLEELQLFKNKET SVAIEVIEDTKEKRTIIHQA IKSLFPGLETKTEDREGKKYIVAYH AAGKKALANPRKHSWPKSRGSYCHF VLYKENKDTMDAINVLSKYL RVKPNIFSYMGTKDKRAITVQEIAV LKITAQRLAHLNKCLMNFKLGNFSY QKNPLKLGELQGNHFTVVLR NITGTDDQVQQAMNSLKEIGFINYY GMQRFGTTAVPTYQVGRAILQNSWT EVMDLILKPRSGAEKGYLVK CREEWAKTKDPTAALRKLPVKRCVE GQLLRGLSKYGMKNIVSAFGIIPRN NRLMYIHSYQSYVWNNMVSK RIEDYGLKPVPGDLVLKGATATYIE EDDVNNYSIHDVVMPLPGFDVIYPK HKIQEAYREMLTADNLDIDN MRHKIRDYSLSGAYRKIIIRPQNVS WEVVAYDDPKIPLFNTDVDNLEGKT PPVFASEGKYRALKMDFSLP
  PSTYATMAIREVLKMDTSIKNQTQL NTTWLRTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PUS7 pseudouridine synthase 7 [ Homo sapiens (human) ]
• Official Symbol: PUS7
• Synonyms: IDDABS
• Gene ID: 54517
• mRNA Refseq: NM_019042.5
• Protein Refseq: NP_061915.2
• MIM: 616261
• UniProt ID: Q96PZ0