Recombinant Full Length Human RAD52 Protein, C-Flag-tagged

• Cat.No.: RAD52-1565HFL
• Product Overview: Recombinant Full Length Human RAD52 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46 kDa
• AA Sequence: MSGTEEAILGGRDSHPAAGGGSVLC FGQCQYTAEEYQAIQKALRQRLGPE YISSRMAGGGQKVCYIEGHR VINLANEMFGYNGWAHSITQQNVDF VDLNNGKFYVGVCAFVRVQLKDGSY HEDVGYGVSEGLKSKALSLE KARKEAVTDGLKRALRSFGNALGNC ILDKDYLRSLNKLPRQLPLEVDLTK AKRQDLEPSVEEARYNSCRP NMALGHPQLQQVTSPSRPSHAVIPA DQDCSSRSLSSSAVESEATHQRKLR QKQLQQQFRERMEKQQVRVS TPSAEKSEAAPPAPPVTHSTPVTVS EPLLEKDFLAGVTQELIKTLEDNSE KWAVTPDAGDGVVKPSSRAD PAQTSDTLALNNQMVTQNRTPHSVC HQKPQAKSGSWDLQTYSADQRTTGN WESHRKSQDMKKRKYDPSTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination

Gene Information

• Gene Name: RAD52 RAD52 homolog, DNA repair protein [ Homo sapiens (human) ]
• Official Symbol: RAD52
• Synonyms: RP11-359B12.2
• Gene ID: 5893
• mRNA Refseq: NM_134424.4
• Protein Refseq: NP_602296.2
• MIM: 600392
• UniProt ID: P43351