Recombinant Full Length Human REN, His-tagged

• Cat.No.: REN-317H
• Product Overview: Recombinant Full Length Human REN(NP_000528.1)(Met 1-Arg 406) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-406 aa
• Form: Lyophilized from sterile 25mM MES, 150mM NaCl, pH6.5.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human RENIN-His at 10 μg/ml (100 μl/well) can bind biotinylated human AGT-His with a linear range of 0.156-5.0 μg/ml.
• Molecular Mass: The secreted recombinant human Renin is a monomeric protein after cleavage of the signal peptide. The form consists of 394 amino acids and predictes a molecular mass of 43.7 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 50 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000528.1
• Unit ID: P00797
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P00797

Gene Information

• Gene Name: REN renin [ Homo sapiens ]
• Official Symbol: REN
• Synonyms: REN; renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme; HNFJ2; FLJ10761
• Gene ID: 5972
• mRNA Refseq: NM_000537
• protein Refseq: NP_000528
• MIM: 179820