Recombinant Full Length Human REN, His tagged

• Cat.No.: REN-317H
• Product Overview: Recombinant Human Renin (NP_000528.1)(Met 1-Arg 406) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg406
• Form: Lyophilized from sterile 25mM MES, 150mM NaCl, pH6.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human Renin is a monomeric protein after cleavage of the signal peptide. The pro form consists of 394 amino acids and predictes a molecular mass of 43.7 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 50 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: REN renin [ Homo sapiens ]
• Official Symbol: REN
• Synonyms: REN; renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme; HNFJ2; FLJ10761
• Gene ID: 5972
• mRNA Refseq: NM_000537
• Protein Refseq: NP_000528
• MIM: 179820
• UniProt ID: P00797