Recombinant Full Length Human RIPK1, GST-tagged

• Cat.No.: RIPK1-7018H
• Product Overview: Full-length recombinant human RIPK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: RIPK1 or Receptor Interacting Protein Kinase 1 is a serine/threonine kinase that was originally identified as interacting with the cytoplasmic domain of FAS. RIPK1 has been deemed as an important element in the signal transduction machinery that mediates programmed cell death. RIPK1 has been shown to interact with TRADD, TRAF1 TRAF2 and TRAF3 and TRADD can act as an adaptor protein to recruit RIPK1 to the TNFR1 complex in a TNF-dependent process. TNFα is capable of activating the noncanonical NF-κB pathway, but this activation of this pathway is negatively regulated by RIPK1.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~108 kDa
• Protein length: 1-671 a.a.
• Purity: >70%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.05 μg/μl
• Publication:
  Genomic instability causes HGF gene activation in colon cancer cells, promoting their resistance to necroptosis (2015)
  Hepatocyte Growth Factor Gene Mutation in Human Colorectal Cancer: Causes and Consequences. (2014)

Gene Information

• Gene Name: RIPK1 receptor (TNFRSF)-interacting serine-threonine kinase 1 [ Homo sapiens ]
• Official Symbol: RIPK1
• Synonyms: RIPK1; receptor (TNFRSF)-interacting serine-threonine kinase 1; receptor-interacting serine/threonine-protein kinase 1; RIP; RIP-1; cell death protein RIP; receptor interacting protein; receptor-interacting protein 1; serine/threonine-protein kinase RIP; RIP1; FLJ39204
• Gene ID: 8737
• mRNA Refseq: NM_003804
• Protein Refseq: NP_003795
• MIM: 603453
• UniProt ID: Q13546
• Chromosome Location: 6p25.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of Pro-Caspase 8, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis Modulation by HSP70, organism-specific biosystem
• Function: ATP binding; death domain binding; death receptor binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Reviews

02/26/2022

The proteins accepted were of very high purity and were put into experiments with satisfactory results.

03/14/2021

Customer service was patient, prompt and accurate in answering my queries.

11/23/2020

Prompt delivery, which helped me to obtain satisfactory experimental results in a short time.

Q&As

What is the relationship between RIPK1 and necrotic apoptosis?

RIPK1 is one of the most critical intracellular signaling factors mediating necrotic apoptosis.

What is the relationship between Necrostatin-1 and RIPK1?

Necrostatin-1 blocks RIPK1 phosphorylation.

What are the effects of inhibiting RIPK1 expression?

Inhibition of RIPK1 reduces astrocyte proliferation and glial scar formation and promotes recovery of brain function.

RIPK1 is closely associated with the study of which diseases?

RIPK1 is closely related to the study of stroke, neurodegenerative diseases, autoimmune diseases and other diseases.