Recombinant Full Length Human SEMA5A Protein, C-Flag-tagged

• Cat.No.: SEMA5A-1015HFL
• Product Overview: Recombinant Full Length Human SEMA5A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 118.1 kDa
• AA Sequence: MKGTCVIAWLFSSLGLWRLAHPEAQ GTTQCQRTEHPVISYKEIGPWLREF RAKNAVDFSQLTFDPGQKEL VVGARNYLFRLQLEDLSLIQAVEWE CDEATKKACYSKGKSKEECQNYIRV LLVGGDRLFTCGTNAFTPVC TNRSLSNLTEIHDQISGMARCPYSP QHNSTALLTAGGELYAATAMDFPGR DPAIYRSLGILPPLRTAQYN SKWLNEPNFVSSYDIGNFTYFFFRE NAVEHDCGKTVFSRAARVCKNDIGG RFLLEDTWTTFMKARLNCSR PGEVPFYYNELQSTFFLPELDLIYG IFTTNVNSIAASAVCVFNLSAIAQA FSGPFKYQENSRSAWLPYPN PNPHFQCGTVDQGLYVNLTERNLQD AQKFILMHEVVQPVTTVPSFMEDNS RFSHVAVDVVQGREALVHII YLATDYGTIKKVRVPLNQTSSSCLL EEIELFPERRREPIRSLQILHSQSV LFVGLREHVVKIPLKRCQFY RTRSTCIGAQDPYCGWDVVMKKCTS LEESLSMTQWEQSISACPTRNLTVD GHFGVWSPWTPCTHTDGSAV GSCLCRTRSCDSPAPQCGGWQCEGP GMEIANCSRNGGWTPWTSWSPCSTT CGIGFQVRQRSCSNPTPRHG GRVCVGQNREERYCNEHLLCPPHMF WTGWGPWERCTAQCGGGIQARRRIC ENGPDCAGCNVEYQSCNTNP CPELKKTTPWTPWTPVNISDNGGHY EQRFRYTCKARLADPNLLEVGRQRI EMRYCSSDGTSGCSTDGLSG DFLRAGRYSAHTVNGAWSAWTSWSQ CSRDCSRGIRNRKRVCNNPEPKYGG MPCLGPSLEYQECNILPCPV DGVWSCWSPWTKCSATCGGGHYMRT RSCSNPAPAYGGDICLGLHTEEALC NTQPCPESWSEWSDWSECEA SGVQVRARQCILLFPMGSQCSGNTT ESRPCVFDSNFIPEVSVARSSSVEE KRCGEFNMFHMIAVGLSSSI LGCLLTLLVYTYCQRYQQQSHDATV IHPVSPAPLNTSITNHINKLDKYDS VEAIKAFNKNNLILEERNKY
  FNPHLTGKTYSNAYFTDLNNYDEYT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Axon guidance

Gene Information

• Gene Name: SEMA5A semaphorin 5A [ Homo sapiens (human) ]
• Official Symbol: SEMA5A
• Synonyms: semF; SEMAF
• Gene ID: 9037
• mRNA Refseq: NM_003966.3
• Protein Refseq: NP_003957.2
• MIM: 609297
• UniProt ID: Q13591