Recombinant Full Length Human SLC12A3 Protein, C-Flag-tagged

• Cat.No.: SLC12A3-1562HFL
• Product Overview: Recombinant Full Length Human SLC12A3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 113.7 kDa
• AA Sequence: MAELPTTETPGDATLCSGRFTISTL LSSDEPSPPAAYDSSHPSHLTHSST FCMRTFGYNTIDVVPTYEHY ANSTQPGEPRKVRPTLADLHSFLKQ EGRHLHALAFDSRPSHEMTDGLVEG EAGTSSEKNPEEPVRFGWVK GVMIRCMLNIWGVILYLRLPWITAQ AGIVLTWIIILLSVTVTSITGLSIS AISTNGKVKSGGTYFLISRS LGPELGGSIGLIFAFANAVGVAMHT VGFAETVRDLLQEYGAPIVDPINDI RIIGVVSVTVLLAISLAGME WESKAQVLFFLVIMVSFANYLVGTL IPPSEDKASKGFFSYRADIFVQNLV PDWRGPDGTFFGMFSIFFPS ATGILAGANISGDLKDPAIAIPKGT LMAIFWTTISYLAISATIGSCVVRD ASGVLNDTVTPGWGACEGLA CSYGWNFTECTQQHSCHYGLINYYQ TMSMVSGFAPLITAGIFGATLSSAL ACLVSAAKVFQCLCEDQLYP LIGFFGKGYGKNKEPVRGYLLAYAI AVAFIIIAELNTIAPIISNFFLCSY ALINFSCFHASITNSPGWRP SFQYYNKWAALFGAIISVVIMFLLT WWAALIAIGVVLFLLLYVIYKKPEV NWGSSVQAGSYNLALSYSVG LNEVEDHIKNYRPQCLVLTGPPNFR PALVDFVGTFTRNLSLMICGHVLIG PHKQRMPELQLIANGHTKWL NKRKIKAFYSDVIAEDLRRGVQILM QAAGLGRMKPNILVVGFKKNWQSAH PATVEDYIGILHDAFDFNYG VCVMRMREGLNVSKMMQAHINPVFD PAEDGKEASARGARPSVSGALDPKA LVKEEQATTIFQSEQGKKTI DIYWLFDDGGLTLLIPYLLGRKRRW SKCKIRVFVGGQINRMDQERKAIIS LLSKFRLGFHEVHILPDINQ NPRAEHTKRFEDMIAPFRLNDGFKD EATVNEMRRDCPWKISDEEITKNRV KSLRQVRLNEIVLDYSRDAA
  LIVITLPIGRKGKCPSSLYMAWLET LSQDLRPPVILIRGNQENVLTFYCQ TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: SLC12A3 solute carrier family 12 member 3 [ Homo sapiens (human) ]
• Official Symbol: SLC12A3
• Synonyms: NCC; TSC; NCCT
• Gene ID: 6559
• mRNA Refseq: NM_000339.3
• Protein Refseq: NP_000330.3
• MIM: 600968
• UniProt ID: P55017