Recombinant Full Length Human TCP1 Protein, C-Flag-tagged

• Cat.No.: TCP1-1996HFL
• Product Overview: Recombinant Full Length Human TCP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 60.2 kDa
• AA Sequence: MEGPLSVFGDRSTGETIRSQNVMAA ASIANIVKSSLGPVGLDKMLVDDIG DVTITNDGATILKLLEVEHP AAKVLCELADLQDKEVGDGTTSVVI IAAELLKNADELVKQKIHPTSVISG YRLACKEAVRYINENLIVNT DELGRDCLINAAKTSMSSKIIGING DFFANMVVDAVLAIKYTDIRGQPRY PVNSVNILKAHGRSQMESML ISGYALNCVVGSQGMPKRIVNAKIA CLDFSLQKTKMKLGVQVVITDPEKL DQIRQRESDITKERIQKILA TGANVILTTGGIDDMCLKYFVEAGA MAVRRVLKRDLKRIAKASGATILST LANLEGEETFEAAMLGQAEE VVQERICDDELILIKNTKARTSASI ILRGANDFMCDEMERSLHDALCVVK RVLESKSVVPGGGAVEAALS IYLENYATSMGSREQLAIAEFARSL LVIPNTLAVNAAQDSTDLVAKLRAF HNEAQVNPERKNLKWIGLDL SNGKPRDNKQAGVFEPTIVKVKSLK FATEAAITILRIDDLIKLHPESKDD KHGSYEDAVHSGALND TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: TCP1 t-complex 1 [ Homo sapiens (human) ]
• Official Symbol: TCP1
• Synonyms: CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha
• Gene ID: 6950
• mRNA Refseq: NM_030752.3
• Protein Refseq: NP_110379.2
• MIM: 186980
• UniProt ID: P17987