Recombinant Full Length Human TIMELESS Protein, C-Flag-tagged

• Cat.No.: TIMELESS-1251HFL
• Product Overview: Recombinant Full Length Human TIMELESS Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 138.5 kDa
• AA Sequence: MDLHMMNCELLATCSALGYLEGDTY HKEPDCLESVKDLIRYLRHEDETRD VRQQLGAAQILQSDLLPILT QHHQDKPLFDAVIRLMVNLTQPALL CFGNLPKEPSFRHHFLQVLTYLQAY KEAFASEKAFGVLSETLYEL LQLGWEERQEEDNLLIERILLLVRN ILHVPADLDQEKKIDDDASAHDQLL WAIHLSGLDDLLLFLASSSA EEQWSLHVLEIVSLMFRDQNPEQLA GVGQGRLAQERSADFAELEVLRQRE MAEKKTRALQRGNRHSRFGG SYIVQGLKSIGERDLIFHKGLHNLR NYSSDLGKQPKKVPKRRQAARELSI QRRSALNVRLFLRDFCSEFL ENCYNRLMGSVKDHLLREKAQQHDE TYYMWALAFFMAFNRAASFRPGLVS ETLSVRTFHFIEQNLTNYYE MMLTDRKEAASWARRMHLALKAYQE LLATVNEMDISPDEAVRESSRIIKN NIFYVMEYRELFLALFRKFD ERCQPRSFLRDLVETTHLFLKMLER FCRSRGNLVVQNKQKKRRKKKKKVL DQAIVSGNVPSSPEEVEAVW PALAEQLQCCAQNSELSMDSVVPFD AASEVPVEEQRAEAMVRIQDCLLAG QAPQALTLLRSAREVWPEGD VFGSQDISPEEEIQLLKQILSAPLP RQQGPEERGAEEEEEEEEEEEEELQ VVQVSEKEFNFLDYLKRFAC STVVRAYVLLLRSYQQNSAHTNHCI VKMLHRLAHDLKMEALLFQLSVFCL FNRLLSDPAAGAYKELVTFA KYILGKFFALAAVNQKAFVELLFWK NTAVVREMTEGYGSLDDRSSSRRAP TWSPEEEAHLRELYLANKDV EGQDVVEAILAHLNTVPRTRKQIIH HLVQMGLADSVKDFQRKGTHIVLWT GDQELELQRLFEEFRDSDDV LGHIMKNITAKRSRARIVDKLLALG LVAERRELYKKRQKKLASSILPNGA ESLKDFCQEDLEEEENLPEE DSEEEEEGGSEAEQVQGSLVLSNEN LGQSLHQEGFSILLLWLQNCLIRAA DDREEDGCSQAVPLVPLTEE NEEAMENEQFQQLLRKLGVRPPASG QETFWRIPAKLSPTQLRRAAASLSQ PEEEQKLQPELQPKVPGEQG SDEEHCKEHRAQALRALLLAHKKKA GLASPEEEDAVGKEPLKAAPKKRQL LDSDEEQEEDEGRNRAPELG
  APGIQKKKRYQIEDDEDDTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors

Gene Information

• Gene Name: TIMELESS timeless circadian regulator [ Homo sapiens (human) ]
• Official Symbol: TIMELESS
• Synonyms: TIM; TIM1; hTIM; FASPS4
• Gene ID: 8914
• mRNA Refseq: NM_003920.5
• Protein Refseq: NP_003911.2
• MIM: 603887
• UniProt ID: Q9UNS1