Recombinant Full Length Human TJP1 Protein, C-Flag-tagged

• Cat.No.: TJP1-384HFL
• Product Overview: Recombinant Full Length Human TJP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 195.3 kDa
• AA Sequence: MSARAAAAKSTAMEETAIWEQHTVT LHRAPGFGFGIAISGGRDNPHFQSG ETSIVISDVLKGGPAEGQLQ ENDRVAMVNGVSMDNVEHAFAVQQL RKSGKNAKITIRRKKKVQIPVSRPD PEPVSDNEEDSYDEEIHDPR SGRSGVVNRRSEKIWPRDRSASRER SLSPRSDRRSVASSQPAKPTKVTLV KSRKNEEYGLRLASHIFVKE ISQDSLAARDGNIQEGDVVLKINGT VTENMSLTDAKTLIERSKGKLKMVV QRDERATLLNVPDLSDSIHS ANASERDDISEIQSLASDHSGRSHD RPPRRSRSRSPDQRSEPSDHSRHSP QQPSNGSLRSRDEERISKPG AVSTPVKHADDHTPKTVEEVTVERN EKQTPSLPEPKPVYAQVGQPDVDLP VSPSDGVLPNSTHEDGILRP SMKLVKFRKGDSVGLRLAGGNDVGI FVAGVLEDSPAAKEGLEEGDQILRV NNVDFTNIIREEAVLFLLDL PKGEEVTILAQKKKDVYRRIVESDV GDSFYIRTHFEYEKESPYGLSFNKG EVFRVVDTLYNGKLGSWLAI RIGKNHKEVERGIIPNKNRAEQLAS VQYTLPKTAGGDRADFWRFRGLRSS KRNLRKSREDLSAQPVQTKF PAYERVVLREAGFLRPVTIFGPIAD VAREKLAREEPDIYQIAKSEPRDAG TDQRSSGIIRLHTIKQIIDQ DKHALLDVTPNAVDRLNYAQWYPIV VFLNPDSKQGVKTMRMRLCPESRKS ARKLYERSHKLRKNNHHLFT TTINLNSMNDGWYGALKEAIQQQQN QLVWVSEGKADGATSDDLDLHDDRL SYLSAPGSEYSMYSTDSRHT SDYEDTDTEGGAYTDQELDETLNDE VGTPPESAITRSSEPVREDSSGMHH ENQTYPPYSPQAQPQPIHRI DSPGFKPASQQKAEASSPVPYLSPE TNPASSTSAVNHNVNLTNVRLEEPT PAPSTSYSPQADSLRTPSTE AAHIMLRDQEPSLSSHVDPTKVYRK DPYPEEMMRQNHVLKQPAVSHPGHR PDKEPNLTYEPQLPYVEKQA SRDLEQPTYRYESSSYTDQFSRNYE HRLRYEDRVPMYEEQWSYYDDKQPY PSRPPFDNQHSQDLDSRQHP EESSERGYFPRFEEPAPLSYDSRPR YEQAPRASALRHEEQPAPGYDTHGR LRPEAQPHPSAGPKPAESKQ YFEQYSRSYEQVPPQGFTSRAGHFE PLHGAAAVPPLIPSSQHKPEALPSN TKPLPPPPTQTEEEEDPAMK PQSVLTRVKMFENKRSASLETKKDV NDTGSFKPPEVASKPSGAPIIGPKP TSQNQFSEHDKTLYRIPEPQ KPQLKPPEDIVRSNHYDPEEDEEYY RKQLSYFDRRSFENKPPAHIAASHL SEPAKPAHSQNQSNFSSYSS KGKPPEADGVDRSFGEKRYEPIQAT PPPPPLPSQYAQPSQPVTSASLHIH SKGAHGEGNSVSLDFQNSLV SKPDPPPSQNKPATFRPPNREDTAQ AAFYPQKSFPDKAPVNGTEQTQKTV TPAYNRFTPKPYTSSARPFE RKFESPKFNHNLLPSETAHKPDLSS KTPTSPKTLVKSHSLAQPPEFDSGV ETFSIHAEKPKYQINNISTV PKAIPVSPSAVEEDEDEDGHTVVAT ARGIFNSNGGVLSSIETGVSIIIPQ GAIPEGVEQEIYFKVCRDNS ILPPLDKEKGETLLSPLVMCGPHGL KFLKPVELRLPHCDPKTWQNKCLPG DPNYLVGANCVSVLIDHFTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection

Gene Information

• Gene Name: TJP1 tight junction protein 1 [ Homo sapiens (human) ]
• Official Symbol: TJP1
• Synonyms: ZO-1
• Gene ID: 7082
• mRNA Refseq: NM_003257.5
• Protein Refseq: NP_003248.3
• MIM: 601009
• UniProt ID: Q07157