Recombinant Full Length Human TRPM4 Protein, C-Flag-tagged

• Cat.No.: TRPM4-1882HFL
• Product Overview: Recombinant Full Length Human TRPM4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 134.1 kDa
• AA Sequence: MVVPEKEQSWIPKIFKKKTCTTFIV DSTDPGGTLCQCGRPRTAHPAVAME DAFGAAVVTVWDSDAHTTEK PTDAYGELDFTGAGRKHSNFLRLSD RTDPAAVYSLVTRTWGFRAPNLVVS VLGGSGGPVLQTWLQDLLRR GLVRAAQSTGAWIVTGGLHTGIGRH VGVAVRDHQMASTGGTKVVAMGVAP WGVVRNRDTLINPKGSFPAR YRWRGDPEDGVQFPLDYNYSAFFLV DDGTHGCLGGENRFRLRLESYISQQ KTGVGGTGIDIPVLLLLIDG DEKMLTRIENATQAQLPCLLVAGSG GAADCLAETLEDTLAPGSGGARQGE ARDRIRRFFPKGDLEVLQAQ VERIMTRKELLTVYSSEDGSEEFET IVLKALVKACGSSEASAYLDELRLA VAWNRVDIAQSELFRGDIQW RSFHLEASLMDALLNDRPEFVRLLI SHGLSLGHFLTPMRLAQLYSAAPSN SLIRNLLDQASHSAGTKAPA LKGGAAELRPPDVGHVLRMLLGKMC APRYPSGGAWDPHPGQGFGESMYLL SDKATSPLSLDAGLGQAPWS DLLLWALLLNRAQMAMYFWEMGSNA VSSALGACLLLRVMARLEPDAEEAA RRKDLAFKFEGMGVDLFGEC YRSSEVRAARLLLRRCPLWGDATCL QLAMQADARAFFAQDGVQSLLTQKW WGDMASTTPIWALVLAFFCP PLIYTRLITFRKSEEEPTREELEFD MDSVINGEGPVGTADPAEKTPLGVP RQSGRPGCCGGRCGGRRCLR RWFHFWGAPVTIFMGNVVSYLLFLL LFSRVLLVDFQPAPPGSLELLLYFW AFTLLCEELRQGLSGGGGSL ASGGPGPGHASLSQRLRLYLADSWN QCDLVALTCFLLGVGCRLTPGLYHL GRTVLCIDFMVFTVRLLHIF TVNKQLGPKIVIVSKMMKDVFFFLF FLGVWLVAYGVATEGLLRPRDSDFP SILRRVFYRPYLQIFGQIPQ EDMDVALMEHSNCSSEPGFWAHPPG AQAGTCVSQYANWLVVLLLVIFLLV ANILLVNLLIAMFSYTFGKV QGNSDLYWKAQRYRLIREFHSRPAL APPFIVISHLRLLLRQLCRRPRSPQ PSSPALEHFRVYLSKEAERK LLTWESVHKENFLLARARDKRESDS ERLKRTSQKVDLALKQLGHIREYEQ RLKVLEREVQQCSRVLGWVA EALSRSALLPPGGPPPPDLPGSKD TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane

Gene Information

• Gene Name: TRPM4 transient receptor potential cation channel subfamily M member 4 [ Homo sapiens (human) ]
• Official Symbol: TRPM4
• Synonyms: EKVP6; LTrpC4; PFHB1B; TRPM4B; hTRPM4
• Gene ID: 54795
• mRNA Refseq: NM_017636.4
• Protein Refseq: NP_060106.2
• MIM: 606936
• UniProt ID: Q8TD43