| Species : |
Human |
| Source : |
E.coli |
| Tag : |
HA |
| Protein Length : |
1-76aa |
| Description : |
Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. Ubiquitination can affect proteins in many ways; it can signal for their degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. HA-Ubiquitin-Vinyl Methyl-Sulfone is a potent, irreversible, and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Vinyl Sulfone group. It targets three of the four major DUB families: UCH (Ubiquitin c-terminal hydrolases), USP (Ubiquitin Specific Proteases), OTU (Ovarian Tumor Proteases), and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited. |
| Tag : |
HA |
| Conjugation/Label : |
Vinyl Methyl Ester |
| Molecular Mass : |
10.1 kDa |
| Purity : |
>97% by LCMS |
| Usage : |
Reaction conditions will need to be optimized for each application. We recommend an initial HA-Ubiquitin-VME concentration of 1-5 μM. |
| Applications : |
HA-Ub-Vinyl Methyl ester can be used for activity profiling experiments and using the HA-Tag conveniently for Immunoprecipitations in search for new or novel enzymes reacting with the c-terminal warhead. |
| Notes : |
For Research Use Only, Not For Use In Humans. |
| Storage : |
At -80 centigrade, Avoid multiple freeze/thaw cycles. |
| Storage Buffer : |
50mM MES pH 6.0, 100mM NaCl |
| Concentration : |
400 μM, 4.0 mg/mL |
