Recombinant Full Length Human UHRF1 Protein, C-Flag-tagged

• Cat.No.: UHRF1-1289HFL
• Product Overview: Recombinant Full Length Human UHRF1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 90.9 kDa
• AA Sequence: MGVFAVPPLSSDTMWIQVRTMDGRQ THTVDSLSRLTKVEELRRKIQELFH VEPGLQRLFYRGKQMEDGHT LFDYEVRLNDTIQLLVRQSLVLPHS TKERDSELSDTDSGCCLGQSESDKS STHGEAAAETDSRPADEDMW DETELGLYKVNEYVDARDTNMGAWF EAQVVRVTRKAPSRDEPCSSTSRPA LEEDVIYHVKYDDYPENGVV QMNSRDVRARARTIIKWQDLEVGQV VMLNYNPDNPKERGFWYDAEISRKR ETRTARELYANVVLGDDSLN DCRIIFVDEVFKIERPGEGSPMVDN PMRRKSGPSCKHCKDDVNRLCRVCA CHLCGGRQDPDKQLMCDECD MAFHIYCLDPPLSSVPSEDEWYCPE CRNDASEVVLAGERLRESKKKAKMA SATSSSQRDWGKGMACVGRT KECTIVPSNHYGPIPGIPVGTMWRF RVQVSESGVHRPHVAGIHGRSNDGA YSLVLAGGYEDDVDHGNFFT YTGSGGRDLSGNKRTAEQSCDQKLT NTNRALALNCFAPINDQEGAEAKDW RSGKPVRVVRNVKGGKNSKY APAEGNRYDGIYKVVKYWPEKGKSG FLVWRYLLRRDDDEPGPWTKEGKDR IKKLGLTMQYPEGYLEALAN REREKENSKREEEEQQEGGFASPRT GKGKWKRKSAGGGPSRAGSPRRTSK KTKVEPYSLTAQQSSLIRED KSNAKLWNEVLASLKDRPASGSPFQ LFLSKVEETFQCICCQELVFRPITT VCQHNVCKDCLDRSFRAQVF
  SCPACRYDLGRSYAMQVNQPLQTVL NQLFPGYGNGRTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors

Gene Information

• Gene Name: UHRF1 ubiquitin like with PHD and ring finger domains 1 [ Homo sapiens (human) ]
• Official Symbol: UHRF1
• Synonyms: Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
• Gene ID: 29128
• mRNA Refseq: NM_013282.5
• Protein Refseq: NP_037414.3
• MIM: 607990
• UniProt ID: Q96T88