Recombinant Full Length Human UPF2 Protein, C-Flag-tagged

• Cat.No.: UPF2-1875HFL
• Product Overview: Recombinant Full Length Human UPF2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 147.6 kDa
• AA Sequence: MPAERKKPASMEEKDSLPNNKEKDC SERRTVSSKERPKDDIKLTAKKEVS KAPEDKKKRLEDDKRKKEDK ERKKKDEEKVKAEEESKKKEEEEKK KHQEEERKKQEEQAKRQQEEEAAAQ MKEKEESIQLHQEAWERHHL RKELRSKNQNAPDSRPEENFFSRLD SSLKKNTAFVKKLKTITEQQRDSLS HDFNGLNLSKYIAEAVASIV EAKLKISDVNCAVHLCSLFHQRYAD FAPSLLQVWKKHFEARKEEKTPNIT KLRTDLRFIAELTIVGIFTD KEGLSLIYEQLKNIINADRESHTHV SVVISFCRHCGDDIAGLVPRKVKSA AEKFNLSFPPSEIISPEKQQ PFQNLLKEYFTSLTKHLKRDHRELQ NTERQNRRILHSKGELSEDRHKQYE EFAMSYQKLLANSQSLADLL DENMPDLPQDKPTPEEHGPGIDIFT PGKPGEYDLEGGIWEDEDARNFYEN LIDLKAFVPAILFKDNEKSC QNKESNKDDTKEAKESKENKEVSSP DDLELELENLEINDDTLELEGGDEA EDLTKKLLDEQEQEDEEAST GSHLKLIVDAFLQQLPNCVNRDLID KAAMDFCMNMNTKANRKKLVRALFI VPRQRLDLLPFYARLVATLH PCMSDVAEDLCSMLRGDFRFHVRKK DQINIETKNKTVRFIGELTKFKMFT KNDTLHCLKMLLSDFSHHHI EMACTLLETCGRFLFRSPESHLRTS VLLEQMMRKKQAMHLDARYVTMVEN AYYYCNPPPAEKTVKKKRPP LQEYVRKLLYKDLSKVTTEKVLRQM RKLPWQDQEVKDYVICCMINIWNVK YNSIHCVANLLAGLVLYQED VGIHVVDGVLEDIRLGMEVNQPKFN QRRISSAKFLGELYNYRMVESAVIF RTLYSFTSFGVNPDGSPSSL DPPEHLFRIRLVCTILDTCGQYFDR GSSKRKLDCFLVYFQRYVWWKKSLE VWTKDHPFPIDIDYMISDTL ELLRPKIKLCNSLEESIRQVQDLER EFLIKLGLVNDKDSKDSMTEGENLE EDEEEEEGGAETEEQSGNES EVNEPEEEEGSDNDDDEGEEEEEEN TDYLTDSNKENETDEENTEVMIKGG GLKHVPCVEDEDFIQALDKM MLENLQQRSGESVKVHQLDVAIPLH LKSQLRKGPPLGGGEGEAESADTMP FVMLTRKGNKQQFKILNVPM SSQLAANHWNQQQAEQEERMRMKKL TLDINERQEQEDYQEMLQSLAQRPA PANTNRERRPRYQHPKGAPN ADLIFKTGGRRR TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: UPF2 UPF2 regulator of nonsense mediated mRNA decay [ Homo sapiens (human) ]
• Official Symbol: UPF2
• Synonyms: HUPF2; RENT2; smg-3
• Gene ID: 26019
• mRNA Refseq: NM_080599.3
• Protein Refseq: NP_542166.1
• MIM: 605529
• UniProt ID: Q9HAU5