Recombinant Full Length Human USO1 Protein, C-Flag-tagged

• Cat.No.: USO1-1756HFL
• Product Overview: Recombinant Full Length Human USO1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 107.7 kDa
• AA Sequence: MNFLRGVMGGQSAGPQHTEAETIQK LCDRVASSTLLDDRRNAVRALKSLS KKYRLEVGIQAMEHLIHVLQ TDRSDSEIIGYALDILYNIISNEEE EEVEENSTRQSEDLGSQFTEIFIKQ QENVTLLLSLLEEFDFHVRW PGVKLLTSLLKQLGPQVQQIILVSP MGVSRLMDLLADSREVIRNDGVLLL QALTRSNGAIQKIVAFENAF ERLLDIISEEGNSDGGIVVEDCLIL LQNLLKNNNSNQNFFKEGSYIQRMK PWFEVGDENSGWSAQKVTNL HLMLQLVRVLVSPTNPPGATSSCQK AMFQCGLLQQLCTILMATGVPADIL TETINTVSEVIRGCQVNQDY FASVNAPSNPPRPAIVVLLMSMVNE RQPFVLRCAVLYCFQCFLYKNQKGQ GEIVSTLLPSTIDATGNSVS AGQLLCGGLFSTDSLSNWCAAVALA HALQENATQKEQLLRVQLATSIGNP PVSLLQQCTNILSQGSKIQT RVGLLMLLCTWLSNCPIAVTHFLHN SANVPFLTGQIAENLGEEEQLVQGL CALLLGISIYFNDNSLESYM KEKLKQLIEKRIGKENFIEKLGFIS KHELYSRASQKPQPNFPSPEYMIFD HEFTKLVKELEGVITKAIYK SSEEDKKEEEVKKTLEQHDNIVTHY KNMIREQDLQLEELRQQVSTLKCQN EQLQTAVTQQVSQIQQHKDQ YNLLKIQLGKDNQHQGSYSEGAQMN GIQPEEIGRLREEIEELKRNQELLQ SQLTEKDSMIENMKSSQTSG TNEQSSAIVSARDSEQVAELKQELA TLKSQLNSQSVEITKLQTEKQELLQ KTEAFAKSVEVQGETETIIA TKTTDVEGRLSALLQETKELKNEIK ALSEERTAIKEQLDSSNSTIAILQT EKDKLELEITDSKKEQDDLL
  VLLADQDQKILSLKNKLKDLGHPVE EEDELESGDQEDEDDESEDPGKDLD HITRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: USO1 USO1 vesicle transport factor [ Homo sapiens (human) ]
• Official Symbol: USO1
• Synonyms: TAP; VDP; P115
• Gene ID: 8615
• mRNA Refseq: NM_003715.4
• Protein Refseq: NP_003706.2
• MIM: 603344
• UniProt ID: O60763