Recombinant Full Length Human VIM Protein, C-Flag-tagged

• Cat.No.: VIM-17HFL
• Product Overview: Recombinant Full Length Human VIM Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 53.5 kDa
• AA Sequence: MSTRSVSSSSYRRMFGGPGTASRPS SSRSYVTTSTRTYSLGSALRPSTSR SLYASSPGGVYATRSSAVRL RSSVPGVRLLQDSVDFSLADAINTE FKNTRTNEKVELQELNDRFANYIDK VRFLEQQNKILLAELEQLKG QGKSRLGDLYEEEMRELRRQVDQLT NDKARVEVERDNLAEDIMRLREKLQ EEMLQREEAENTLQSFRQDV DNASLARLDLERKVESLQEEIAFLK KLHEEEIQELQAQIQEQHVQIDVDV SKPDLTAALRDVRQQYESVA AKNLQEAEEWYKSKFADLSEAANRN NDALRQAKQESTEYRRQVQSLTCEV DALKGTNESLERQMREMEEN FAVEAANYQDTIGRLQDEIQNMKEE MARHLREYQDLLNVKMALDIEIATY RKLLEGEESRISLPLPNFSS
  LNLRETNLDSLPLVDTHSKRTLLIK TVETRDGQVINETSQHHDDLETRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: ES Cell Differentiation/IPS

Gene Information

• Gene Name: VIM vimentin [ Homo sapiens (human) ]
• Official Symbol: VIM
• Synonyms: CTRCT30; HEL113
• Gene ID: 7431
• mRNA Refseq: NM_003380.5
• Protein Refseq: NP_003371.2
• MIM: 193060
• UniProt ID: P08670