Recombinant Full Length Human XRCC1 Protein, C-Flag-tagged

• Cat.No.: XRCC1-778HFL
• Product Overview: Recombinant Full Length Human XRCC1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 69.3 kDa
• AA Sequence: MPEIRLRHVVSCSSQDSTHCAENLL KADTYRKWRAAKAGEKTISVVLQLE KEEQIHSVDIGNDGSAFVEV LVGSSAGGAGEQDYEVLLVTSSFMS PSESRSGSNPNRVRMFGPDKLVRAA AEKRWDRVKIVCSQPYSKDS PFGLSFVRFHSPPDKDEAEAPSQKV TVTKLGQFRVKEEDESANSLRPGAL FFSRINKTSPVTASDPAGPS YAAATLQASSAASSASPVSRAIGST SKPQESPKGKRKLDLNQEEKKTPSK PPAQLSPSVPKRPKLPAPTR TPATAPVPARAQGAVTGKPRGEGTE PRRPRAGPEELGKILQGVVVVLSGF QNPFRSELRDKALELGAKYR PDWTRDSTHLICAFANTPKYSQVLG LGGRIVRKEWVLDCHRMRRRLPSQR YLMAGPGSSSEEDEASHSGG SGDEAPKLPQKQPQTKTKPTQAAGP SSPQKPPTPEETKAASPVLQEDIDI EGVQSEGQDNGAEDSGDTED ELRRVAEQKEHRLPPGQEENGEDPY AGSTDENTDSEEHQEPPDLPVPELP DFFQGKHFFLYGEFPGDERR KLIRYVTAFNGELEDYMSDRVQFVI TAQEWDPSFEEALMDNPSLAFVRPR WIYSCNEKQKLLPHQLYGVV
  PQATRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair

Gene Information

• Gene Name: XRCC1 X-ray repair cross complementing 1 [ Homo sapiens (human) ]
• Official Symbol: XRCC1
• Synonyms: RCC; SCAR26
• Gene ID: 7515
• mRNA Refseq: NM_006297.3
• Protein Refseq: NP_006288.2
• MIM: 194360
• UniProt ID: P18887