Recombinant Full Length Pongo Abelii Dihydroorotate Dehydrogenase (Quinone), Mitochondrial(Dhodh) Protein, His-Tagged

• Cat.No.: RFL28959PF
• Product Overview: Recombinant Full Length Pongo abelii Dihydroorotate dehydrogenase (quinone), mitochondrial(DHODH) Protein (Q5R6X6) (1-395aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-395)
• AA Sequence: MAWRHLKKRAQDAVVILGGGGLLFA SYLMATGDERFYAEHLMPTLQGLLD PESAHRLAVR FTSLGLLPRARFQDSDMLEVRVLGH KFRNPVGIAAGFDKHGEAVDGLYKM GFGFVEIGSV TPKPQEGNPRPRVFRLPEDQAVINR YGFNSHGLSVVEHRLRARQQKQAKL TEDGLPLGVN LGKNKTSVDAAEDYAEGVRVLGPLA DCLVVNVSSPNTAGLRNLQGKAELR RLLTKVLQER DGLRGVHRPAVLVKIAPDLTSQDKE DIASVVKELGIDGLIVTNTTVSRPA GLQGALRSET GGLSGKPPRDLSTETIREMYALTQG RVPIIGVGGVSSGQDALEKIRAGAS LVQLYTALTF WGPPVVGKVKRELEALLKEQGFCRV TDAIGADHRR
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: DHODH
• Synonyms: DHODH; Dihydroorotate dehydrogenase; quinone, mitochondrial; DHOdehase; Dihydroorotate oxidase
• UniProt ID: Q5R6X6

Gene Information

• Gene Name: DHODH
• Synonyms: DHODH; Dihydroorotate dehydrogenase; quinone, mitochondrial; DHOdehase; Dihydroorotate oxidase
• UniProt ID: Q5R6X6

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Q&As

How does DHODH contribute to pyrimidine biosynthesis?

It catalyzes a key step in the pyrimidine biosynthesis pathway, converting dihydroorotate to orotate.

What is the primary role of DHODH in cellular metabolism?

DHODH, dihydroorotate dehydrogenase, is crucial for the de novo synthesis of pyrimidines, essential for DNA and RNA synthesis.

How does DHODH interact with other enzymes in the pyrimidine biosynthesis pathway?

DHODH collaborates with other enzymes in pyrimidine biosynthesis, playing a role in nucleotide metabolism.

What role does DHODH play in cell proliferation and growth?

DHODH is important for cell proliferation, as pyrimidine synthesis is essential for DNA replication and cell growth.

How do genetic variations in DHODH affect its function in nucleotide synthesis?

Genetic mutations in DHODH can disrupt nucleotide synthesis, affecting cellular replication and function.

What is the impact of altered DHODH activity on autoimmune diseases?

Altered DHODH activity is linked with autoimmune conditions, as it affects lymphocyte proliferation and function.

What potential therapeutic applications arise from targeting DHODH in disease treatment?

Targeting DHODH in therapies could be effective in treating autoimmune diseases and certain cancers by modulating pyrimidine synthesis.