Recombinant Full Length Zebrafish DNMT5 Protein, C-His tagged

Cat.No. : DNMT5-2683Z
Product Overview : Recombinant Full Length Zebrafish DNMT5 Protein with His tag was expressed in HEK293.
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Description : Predicted to enable DNA (cytosine-5-)-methyltransferase activity; DNA binding activity; and metal ion binding activity. Predicted to act upstream of or within DNA methylation and regulation of gene expression. Predicted to be located in nucleus. Is expressed in several structures, including brain; digestive system; head; musculature system; and pleuroperitoneal region. Human ortholog(s) of this gene implicated in several diseases, including acute myeloid leukemia; autoimmune disease (multiple); carcinoma (multiple); immunodeficiency-centromeric instability-facial anomalies syndrome 1; and thymoma. Orthologous to human DNMT3B (DNA methyltransferase 3 beta) and DNMT3L (DNA methyltransferase 3 like).
Source : HEK293
Species : Zebrafish
Tag : C-His
Molecular Mass : The protein has a calculated MW of 148 kDa.
AA Sequence : MPRRRSSRLRCPKQQIKNGKQNSNN AFTCNPATMCFIKRYASSTSDCGSV SKNPALDQKYWEDITAICRQTPYCL YLYHGVEIENDKKASVLLLGFFDED TGKNKIRLLDVVYPTKESTEDICNY ILDLLRKIGIPISNMAILYSDFPDH EHLVAGLQLMKAEIVSLCGLTDLTG QVCHSGVEKIEFSDLILNLIIEIYK HFPSFPADLQALLEDVEGSDIDNLT SQCSLFWRIIKKISLAWSHLEKYFG SLDTEEEAVCLLLEDPKIKLNVLFL THALQPLCDFQEIIDQGASVLQLLQ DASKLLRLYTQSFLRPKAAEYFHRR GKTSLVQETVGHLPRGEVAVGEQAA DFLQQHSEELSDYLETFHRSIISFY TTVTVNIVKRLPLPDSTLRNLSLVL SPGKKLEVTGKMVQDLGIGFGVCSE NVSLLKDEFLEYQFIDESDAQPADQ SMKQYWKTELRIMGKASSFGKVILS LLALPTTLKKEQNFERLFQHTDNIK RKKLKDCKKHVSEDDVTDSSSYKSA PSHLSPETQDVIDLGEEEDVEPMEV EDTLPVSDLNTESQNDTKGNCPNVS SNDDDDKCSSSSSHDDYSSSDDDDD GDVIWREKGKGNSCHNNEQTKNPYK GSYIVGEMVWGPLEGFGLWPGLVQS WDSRKPCGSMRKVFFFGNSMQSEIQ ADDLTPFFSFAKCFSSNSFATVATY KDAIFNSLQVASRRSRMFFSPDTDS MDELLRVMLNWAFGGFKPSGEDGLR PQSECNELVFTPRSVNGNDGPSSNF KDHREKLFNLTVSLNKLPESLDLHR GSIDIGKTHVYRKFMLSKRSLRPLQ SREKKYTHWKRYLNDMESMKVDSRQ NSQERYEMVHAFLENKMDIEEFCLS CGSTFVDIIHPLFEGSLCVKCKFNF TETLYRYDEDGYQSYCTVCCSGMEV ILCGHDSCCRCYCVDCLDILVGKGT FKQLKNVDPWTCYLCAPETSSGALK PRHDWSIRVQELFANNSAMQFEPHR VYPSIPADQRRPVRVLSLFDGIATG YLVLRDLGFKVEKYVASEVDEESVT ISMVNHEGKITYVDDVKKITRKHID KWGPFDLLIGGSPCNDLCTVNPGRK GLFEGSGRLFFEYYRLLNVLKPKED DPRPFFWLFENVVLMENRVRADISR FLECNPVLIDAVKVSPAHRARYFWG NIPGMNRPIIASQKDKVSLQDCLDG GRTAKYEKIRTITTRPNILRPGIDD RHYPIVMNGKDDSLWITEIEKIFGF PKHYTDVKNLGRMQRQRVLGKSWSV PVIRHLLAPLKDYFACDEFPAKHHH HHHHH
Endotoxin : < 1 EU/μg by LAL
Purity : > 80% by SDS-PAGE
Storage : Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Concentration : 0.11 mg/mL
Storage Buffer : Sterile PB, 500 mM NaCl, 1% SKL, pH 7.4.
SDS-PAGE: 1. DNMT5-2683Z <br />2. BSA
Gene Name : dnmt3bb.3 DNA (cytosine-5-)-methyltransferase beta, duplicate b.3 [ Danio rerio (zebrafish) ]
Official Symbol : DNMT5
Synonyms : dnmt3bb.3; DNA (cytosine-5-)-methyltransferase beta, duplicate b.3; dnmt5; dnmt3-2; wu:fc08a10; zgc:136325; DNA (cytosine-5-)-methyltransferase beta, duplicate b.3; DNA methyltransferase dnmt5; DNA (cytosine-5-)-methyltransferase 5; dnmt3b4; EC 2.1.1.37
Gene ID : 323723
mRNA Refseq : NM_001020479
Protein Refseq : NP_001018315
UniProt ID : Q588C5

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