| Cat.No. : |
PCRA-1771G |
| Product Overview : |
Recombinant Geobacillus Stearothermophilus (Bacillus stearothermophilus) PCRA Protein (10-289 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Partial. |
| Description : |
DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA. |
| Source : |
E. coli |
| Species : |
Geobacillus Stearothermophilus |
| Tag : |
His-SUMO |
| Form : |
Tris-based buffer,50% glycerol |
| Molecular Mass : |
48.6 kDa |
| Protein length : |
10-289 aa |
| AA Sequence : |
AHLNKEQQEA VRTTEGPLLI MAGAGSGKTR VLTHRIAYLM AEKHVAPWNI LAITFTNKAA REMRERVQSL LGGAAEDVWI STFHSMCVRI LRRDIDRIGI NRNFSILDPT DQLSVMKTIL KEKNIDPKKF EPRTILGTIS AAKNELLPPE QFAKRASTYY EKVVSDVYQE YQQRLLRNHS LDFDDLIMTT IQLFDRVPDV LHYYQYKFQY IHIDEYQDTN RAQYTLVKKL AERFQNICAV GDADQSIYRW RGADIQNILS FERDYPNAKV ILLEQNYRST |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : |
A hardcopy of COA with reconstitution instruction is sent along with the products. |
| Synonyms : |
pcrA; |
| UniProt ID : |
P56255 |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.