Recombinant Geobacillus Stearothermophilus PCRA Protein (10-289 aa), His-SUMO-tagged

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Recombinant Geobacillus Stearothermophilus PCRA Protein (10-289 aa), His-SUMO-tagged

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Cat.No. : PCRA-1771G
Product Overview : Recombinant Geobacillus Stearothermophilus (Bacillus stearothermophilus) PCRA Protein (10-289 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Partial.
Description : DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.
Source : E. coli
Species : Geobacillus Stearothermophilus
Tag : His-SUMO
Form : Tris-based buffer,50% glycerol
Molecular Mass : 48.6 kDa
Protein length : 10-289 aa
AA Sequence : AHLNKEQQEA VRTTEGPLLI MAGAGSGKTR VLTHRIAYLM AEKHVAPWNI LAITFTNKAA REMRERVQSL LGGAAEDVWI STFHSMCVRI LRRDIDRIGI NRNFSILDPT DQLSVMKTIL KEKNIDPKKF EPRTILGTIS AAKNELLPPE QFAKRASTYY EKVVSDVYQE YQQRLLRNHS LDFDDLIMTT IQLFDRVPDV LHYYQYKFQY IHIDEYQDTN RAQYTLVKKL AERFQNICAV GDADQSIYRW RGADIQNILS FERDYPNAKV ILLEQNYRST
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Synonyms : pcrA;
UniProt ID : P56255
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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