Recombinant Geobacillus Stearothermophilus PCRA Protein (10-289 aa), His-SUMO-tagged
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| Cat.No. : | PCRA-1771G |
| Product Overview : | Recombinant Geobacillus Stearothermophilus (Bacillus stearothermophilus) PCRA Protein (10-289 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Partial. |
| Description : | DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA. |
| Source : | E. coli |
| Species : | Geobacillus Stearothermophilus |
| Tag : | His-SUMO |
| Form : | Tris-based buffer,50% glycerol |
| Molecular Mass : | 48.6 kDa |
| Protein length : | 10-289 aa |
| AA Sequence : | AHLNKEQQEA VRTTEGPLLI MAGAGSGKTR VLTHRIAYLM AEKHVAPWNI LAITFTNKAA REMRERVQSL LGGAAEDVWI STFHSMCVRI LRRDIDRIGI NRNFSILDPT DQLSVMKTIL KEKNIDPKKF EPRTILGTIS AAKNELLPPE QFAKRASTYY EKVVSDVYQE YQQRLLRNHS LDFDDLIMTT IQLFDRVPDV LHYYQYKFQY IHIDEYQDTN RAQYTLVKKL AERFQNICAV GDADQSIYRW RGADIQNILS FERDYPNAKV ILLEQNYRST |
| Purity : | > 90% as determined by SDS-PAGE. |
| Notes : | Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : | A hardcopy of COA with reconstitution instruction is sent along with the products. |
| Synonyms : | pcrA; |
| UniProt ID : | P56255 |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. |
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