Active Recombinant Guinea pig Ifng protein, His-tagged

• Cat.No.: Ifng-803G
• Product Overview: Active Recombinant Guinea pig Ifng protein(Q8CGS0)(24~166 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Guinea pig
• Source: E.coli
• Tag: His
• Protein Length: 24-166 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. It has been reported that IFN-γ promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages as an important activator. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 264.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 264.7 cells were incubated in DMEM with IFN-γ (10ng/mL) for 24h, then cells were observed by inverted microscope and iNOS in cell lysates was detected by ELISA.
• Molecular Mass: 18.2kDa
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Ifng interferon gamma [ Cavia porcellus ]
• Official Symbol: Ifng
• Synonyms: IFNG; interferon gamma; interferon, gamma
• Gene ID: 100379568
• mRNA Refseq: NM_001172874
• Protein Refseq: NP_001166345