Recombinant HIV-1/Clade B Nef Protein, His tagged

• Cat.No.: nef-178H
• Product Overview: Recombinant HIV-1 Nef protein with C-terminal 6×His tag was expressed and purified from E.coli.

Specification

• Species: HIV
• Source: E.coli
• Tag: His
• Description: Nef (Negative Regulatory Factor) is a small 27-35 kDa myristoylated protein encoded by primate lentiviruses. These include Human Immunodeficiency Viruses (HIV-1 and HIV-2) and Simian Immunodeficiency Virus (SIV).
• Tag: C-6×His
• Purity: > 90 % by SDS-PAGE
• Applications: Western Blot, ELISA, etc.
• Storage: Store at -20 centigrade; Stable for 3 months from the date of shipment when kept at 4 centigrade. Nonhazardous. No MSDS required
• Concentration: 1 mg/mL
• Storage Buffer: 50 mM Phosphate buffer, pH 7.4, 0.5M NaCl, 0.2M Imidazole, 8M Urea

Gene Information

• Gene Name: nef Nef [ Human immunodeficiency virus 1 ]
• Official Symbol: nef
• Synonyms: nef; Nef; This particular nucleotide sequence has a premature stop codon in place of a well-conserved tryptophan codon at position 8712-8714 that truncates the HIV1 Nef protein sequence to a 123 amino acids-long N-terminal portion (not shown).
• Gene ID: 156110
• Protein Refseq: NP_057857
• UniProt ID: P03406