Recombinant HIV-1 Gag p15

• Cat.No.: Gag p15-92H
• Product Overview: Recombinant HIV-1 Gag p15 was expressed in E. coli.

Specification

• Species: HIV
• Source: E.coli
• Tag: Non
• Description: HIV-1 Gag p15 is processed by digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA.
• Form: 50% glycerol, 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
• Molecular Mass: Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles.
• Purity: Over 90% by SDS-PAGE (CBB staining)
• Applications: 1) It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.2) It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.3) It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.4) It can be used as a standard for the quantitative analysis of HIV-1 p15 antigen.
• Storage: Store at -20ºC
• Concentration: 0.42 mg/ml as measured by BCA method