Recombinant HSV-1 glycoprotein G Antigen, His tagged

• Cat.No.: gG1-073H
• Product Overview: The recombinant gG-1 glycoprotein has been prepared by expressing the mature protein from the US4 gene of HSV-1.

Specification

• Species: HSV1
• Source: P.pastoris
• Tag: His
• Description: gG1 is the recombinant mature glycoprotein G for herpes simplex virus type 1 (HSV-1). The glycoprotein G, an envelope protein of unknown function has been suggested as a prototype antigen for type-specifc HSV-1 serology. Several studies have demonstrated that IgG test using immunoassay or immunoblot based on the specific glycoprotein G is the most accurate method for discrimination HSV-1 and HSV-2.
• Tag: C-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is between markers of 35-45 kDa due to the glycosylation pattern, while relative molecular mass calculated from amino acid sequence and without glycosylation is 19760.72 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7, 0.15 M NaCl and and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 2.95 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Frame, M.C., Marsden,H.S., and McGeoch,D.J. Novel herpes simplex virus type 1 glycoproteins identified by a synthetic oligopeptide from the predicted product of gene US4. 1986. J. gen. Virol. 67:745-751.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26